A semi-quantitative ELISA for detection of Trypanosoma brucei gambiense specific antibodies in serum and cerebrospinal fluid of sleeping sickness patients
A semi-quantitative ELISA for detection of Trypanosoma brucei gambiense specific antibodies in serum and cerebrospinal fluid of sleeping sickness patients
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DOI：
10.1016/s0001-706x(97)00137-x
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发表时间：
1998-05-01
期刊：
影响因子：
2.7
通讯作者：
Van Meirvenne, N
中科院分区：
文献类型：
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作者：
Lejon, V;Büscher, P;Van Meirvenne, N
用结核分枝杆菌表面可变糖蛋白建立的半定量ELISA法以冈比亚为抗原，检测昏睡病患者血清和脑脊液中不同免疫球蛋白亚型抗体。用该方法对28例患者的配对血清和脑脊液标本进行了抗体谱研究。同时测定不同Ig亚型的总浓度。在血清和脑脊液中，血清和脑脊液中的免疫球蛋白G和免疫球蛋白M水平均显著升高，以免疫球蛋白G为主。免疫球蛋白A浓度维持相对正常。血清和脑脊液中检出的抗锥虫抗体主要为IgG1型和IgG3型和IgM型。通过测量血清和脑脊液中免疫球蛋白和锥虫特异性抗体浓度，可以计算鞘内抗体合成，并可能成为确定昏睡病临床分期的一种工具。(C)1998 Elsevier Science B.V.保留所有权利。
A semi-quantitative ELISA, using variable surface glycoprotein of T.b. gambiense as antigen, was developed for the detection of antibodies of different immunoglobulin isotypes in serum and cerebrospinal fluid of sleeping sickness patients. Using the assay, the antibody profiles of paired serum and cerebrospinal fluid samples of 28 patients have been studied. Total concentrations of various Ig isotypes were determined as well. In serum and cerebrospinal fluid a drastic increase in IgG, basically IgG1, as a ell as in IgM levels was observed. The concentration of IgA remained relatively normal. The antitrypanosomal antibodies detected in serum and cerebrospinal fluid were mainly of the IgG (IgG1 and IgG3) and IgM isotypes. Measurement of immunoglobulin and trypanosome specific antibody concentrations in serum and CSF allows calculation of intrathecal antibody synthesis and is a possible tool for determining the clinical stage of sleeping sickness. (C) 1998 Elsevier Science B.V. All rights reserved.