Possible interactions between bacterial diversity, microbial activity and supraglacial hydrology of cryoconite holes in Svalbard
Possible interactions between bacterial diversity, microbial activity and supraglacial hydrology of cryoconite holes in Svalbard
复制标题
DOI：
10.1038/ismej.2010.100
复制
发表时间：
2011-01-01
期刊：
影响因子：
11
通讯作者：
Griffith, Gareth W.
中科院分区：
文献类型：
--
作者：
Edwards, Arwyn;Anesio, Alexandre M.;Griffith, Gareth W.
利用16S rRNA基因座的末端限制性片段长度多态性(T-RFLP)技术，研究了斯瓦尔巴群岛3个北极冰川上低温球洞中高效细菌群落的多样性。克隆文库的构建和测序使这些群落的几个成员得以鉴定，其中变形杆菌占主导地位，其次是蓝藻和拟杆菌。T-RFLP数据显示，与邻近的两个(多热)山谷冰川Midtre Lovenbreen和Vestre Broggerbreen相比，(冷)山谷冰川Aute Broggerbreen上的洞穴中的群落明显不同。这些种群组成与有机质含量、温度和相关微生物群落的代谢活动的差异有关。在两年期间和间隔1公里的采样中，没有观察到冰川内的空间格局。我们推断，地表水文是低温细菌群落发育的一个重要因素。《ISME期刊》(2011年)第5期，第150-160期；doi：10.1038/ismej.2010.100；2010年7月22日在线出版
The diversity of highly active bacterial communities in cryoconite holes on three Arctic glaciers in Svalbard was investigated using terminal restriction fragment length polymorphism (T-RFLP) of the 16S rRNA locus. Construction and sequencing of clone libraries allowed several members of these communities to be identified, with Proteobacteria being the dominant one, followed by Cyanobacteria and Bacteroidetes. T-RFLP data revealed significantly different communities in holes on the (cold) valley glacier Austre Broggerbreen relative to two adjacent (polythermal) valley glaciers, Midtre Lovenbreen and Vestre Broggerbreen. These population compositions correlate with differences in organic matter content, temperature and the metabolic activity of microbial communities concerned. No within-glacier spatial patterns were observed in the communities identified over the 2-year period and with the 1 km-spaced sampling. We infer that surface hydrology is an important factor in the development of cryoconite bacterial communities. The ISME Journal (2011) 5, 150-160; doi:10.1038/ismej.2010.100; published online 22 July 2010