Assessment of genetic diversity and relationship among a collection of US sweet sorghum germplasm by SSR markers
Assessment of genetic diversity and relationship among a collection of US sweet sorghum germplasm by SSR markers
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DOI：
10.1007/s11032-007-9149-z
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发表时间：
2008-05-01
影响因子：
3.1
通讯作者：
Dweikat, I.
中科院分区：
文献类型：
--
作者：
Ali, M. L.;Rajewski, J. F.;Dweikat, I.
甜高粱（Sorghum bicolor L.）高粱是一种人工栽培的高粱，由于其茎中可发酵糖含量高，已被广泛认为是潜在的生物燃料替代来源。已知美国甜高粱中存在糖含量和相关性状的显著变化。本研究的目的是利用SSR标记评估美国甜高粱品种和品系之间的遗传多样性和相互关系，并研究甜高粱种质内糖含量的遗传变异。利用41个SSR标记对68个甜高粱和4个粒用高粱品种和品系进行了基因分型，共产生132个等位基因，平均每个位点产生3.22个等位基因。多态性信息含量（PIC）值，基因多样性的措施，是0.40，范围为0.03-0.87。根据132个SSR等位基因的分离情况估算了遗传相似系数。基于遗传相似性（GS）的聚类分析将72份高粱材料分为10个不同的类群。基于聚类分析的聚类结果与现有的系谱和遗传背景信息吻合良好。揭示了亲本未知品种与亲本已知品种的亲缘关系。本研究鉴定了一批在多个SSR位点上具有多态性的甜高粱材料，这些材料的含糖量也存在显著差异。从这项研究中产生的信息可以用来选择杂交发展的父母，以最大限度地提高糖含量和总生物量，和分离群体的发展，以映射基因控制甜高粱糖含量。
Sweet sorghum (Sorghum bicolor L.) is a type of cultivated sorghums and has been recognized widely as potential alternative source of bio-fuel because of its high fermentable sugar content in the stalk. A substantial variation of sugar content and related traits is known to exist in US sweet sorghum. The objectives of the study were to assess the genetic diversity and relationship among the US sweet sorghum cultivars and lines using SSR markers and to examine the genetic variability within sweet sorghum accessions for sugar content. Sixty-eight sweet sorghum and four grain sorghum cultivars and lines were genotyped with 41 SSR markers that generated 132 alleles with an average of 3.22 alleles per locus. Polymorphism information content (PIC) value, a measure of gene diversity, was 0.40 with a range of 0.03-0.87. The genetic similarity co-efficient was estimated based on the segregation of the 132 SSR alleles. Clustering analysis based on the genetic similarity (GS) grouped the 72 sorghum accessions into 10 distinct clusters. Grouping based on clustering analysis was in good agreement with available pedigree and genetic background information. The study has revealed the genetic relationship of cultivars with unknown parentage to those with known parentage. A number of diverse pairs of sweet sorghum accessions were identified which were polymorphic at many SSR loci and significantly different for sugar content as well. Information generated from this study can be used to select parents for hybrid development to maximize the sugar content and total biomass, and development of segregating populations to map genes controlling sugar content in sweet sorghum.