A panel of eight mRNA signatures improves prognosis prediction of osteosarcoma patients.
A panel of eight mRNA signatures improves prognosis prediction of osteosarcoma patients.
复制标题
DOI：
10.1097/md.0000000000024118
复制
发表时间：
2021-04-09
期刊：
影响因子：
1.6
通讯作者：
Ye Z
中科院分区：
文献类型：
--
作者：
Wu B;Wang Z;Lin N;Yan X;Lv Z;Ying Z;Ye Z
遗传改变对骨肉瘤癌的进展至关重要。本研究调查了一组可以评估骨肉瘤预后的基因特征，这些基因特征基于治疗应用研究以产生有效治疗计划的数据。从治疗应用研究中下载骨肉瘤信使RNA （mRNA）谱和临床数据，生成有效治疗数据库。骨肉瘤患者根据诊断结果分为两组：有和没有转移。比较各组间mrna表达差异。单因素和多因素Cox回归分析确定了一组8个mrna，它们能够将患者分为高风险和低风险组，并且总生存时间显著不同。进一步分析表明，在调整其他临床因素后，8 - mrna特征是一个独立的预后因素。接受者工作特征曲线分析表明，8 - mrna的特征表现良好。利用《基因本体》和《京都基因与基因组百科全书》的资源，对8 - mrna特征的生物学过程和信号通路进行了综述。最后，TCGA分析结果通过基因表达综合数据库中的其他队列进行验证。8 - mrna特征的鉴定不仅提供了骨肉瘤的预后生物标志物，而且为其治疗提供了新的治疗靶点的潜力。
Genetic alterations are vital to the progression of osteosarcoma carcinoma. The present study investigated a panel of gene signatures that could evaluate prognosis in osteosarcoma based on data from the Therapeutically Applicable Research To Generate Effective Treatments initiative. Osteosarcoma messenger RNA (mRNA) profiles and clinical data were downloaded from the therapeutically applicable research to generate effective treatments database. Patients with osteosarcoma were divided into two groups based on findings at diagnosis: with and without metastasis. Differentially expressed mRNAs were compared and analyzed between groups. Univariate and multivariate Cox regression analyses identified a set of eight mRNAs with the ability to classify patients into high-risk and low-risk groups with significantly different overall survival times. Further analysis indicated that the eight-mRNA signature was an independent prognostic factor after adjusting for other clinical factors. Receiver operating characteristic curve analysis demonstrated a good performance of the eight-mRNA signature. Further, the biological processes and signaling pathways of the eight-mRNA signature were reviewed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes resources. Finally, the results of the TCGA analysis were verified by other cohorts from Gene Expression Omnibus database. The identification of an eight-mRNA signature not only provides a prognostic biomarker of osteosarcoma but also offers the potential of novel therapeutic targets for its treatment.