An automatic video instillator for intratracheal instillation in the rat
An automatic video instillator for intratracheal instillation in the rat
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DOI：
10.1258/la.2009.009003
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发表时间：
2010-01-01
影响因子：
2.4
通讯作者：
Song, Chang-Woo
中科院分区：
文献类型：
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作者：
Kim, Jin-Sung;Lee, Bae;Song, Chang-Woo
测试化合物的气管内滴注（ITI）是吸入方法的替代方法，吸入方法需要复杂的气溶胶产生、暴露室和气流监测仪器，用于将动物的肺暴露于测试化合物。对于大鼠的ITI，通常使用喉镜进行气管插管，并且该过程难以执行。因此，我们设计并构建了一种自动视频滴注器（AVI），用于将一定剂量的测试化合物准确输送到大鼠气管中。该装置具有用于图像引导的摄像机探头和用于图像显示的液晶显示器。这两个项目用于可视化喉和气管，以便将管插入喉内，并将滴注管的头端放置在声带之外，以进行供试化合物的ITI。在麻醉大鼠中使用AVI的2 In培训课程后，我们通过将0.25%（w/v）伊文思蓝染料溶液ITI注入30只异氟烷麻醉大鼠的肺中评估了该装置的实用性。在手术完成后立即对20只大鼠进行尸检，并在手术后3天对10只大鼠进行尸检。基于这些检查的结果，我们得出结论，该装置可用于由一名操作员将测试化合物快速、可再现且成功地ITI到大鼠的肺中。
Intratracheal instillation (ITI) of a test compound is an alternative method to inhalation methods that require complex aerosol generation, exposure chambers and airflow monitoring instruments for exposing the lungs of animals to a test compound. For ITI in the rat, a laryngoscope is generally used for endotracheal intubation, and the procedure is difficult to perform. Therefore, we designed and constructed an automatic video instillator (AVI) for the accurate delivery of a dose of a test compound into the trachea of rats. The device has a videocamera probe for image guidance, and a liquid-crystal display for image display. These two items are used to visualize the larynx and trachea for intratracheal insertion of the tubing, and for placing the tip of the instillation tubing beyond the vocal cords for ITI of the test compound. After a 2 In training session on the use of the AVI in an anaesthetized rat, we assessed the utility of the device by ITI of 0.25% (w/v) solution of Evans Blue dye into the lungs of 30 isoflurane-anaesthetized rats. Necropsy examinations were performed on 20 rats immediately after the completion of the procedure, and on 10 rats three days after the procedure. Based on the results of these examinations, we concluded that the device could be used for rapid, reproducible and successful ITI of a test compound into the lungs of a rat by one operator.