INHIBITION OF PHAGOLYSOSOME FUSION IS LOCALIZED TO CHLAMYDIA-PSITTACI-LADEN VACUOLES
INHIBITION OF PHAGOLYSOSOME FUSION IS LOCALIZED TO CHLAMYDIA-PSITTACI-LADEN VACUOLES
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DOI：
10.1128/iai.32.2.889-896.1981
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
WYRICK, PB
中科院分区：
文献类型：
--
作者：
EISSENBERG, LG;WYRICK, PB
C.鹦鹉热部分依赖于生物体阻碍吞噬溶酶体形成的能力。吞噬溶酶体融合的规避可以局限于衣原体负载的空泡或推广到宿主细胞中的所有吞噬体。为了确定这些模式中的哪一种在起作用，通过吖啶橙子和Giemsa染色检查了衣原体初级体和网状体保护酿酒酵母免于在巨噬细胞（小鼠）吞噬溶酶体中降解的能力。在共感染的巨噬细胞和单独感染酵母细胞的巨噬细胞中观察到的融合量之间没有统计学显著差异。这并不依赖于衣原体和酵母细胞之间的某些独特的相互作用，因为确定第二种生物体大肠杆菌的保护作用的活菌计数研究也未能显示在存在衣原体的情况下巨噬细胞对细菌的灭活量有显著差异。鹦鹉因此，吞噬溶酶体融合的抑制作用局限于装载有衣原体的吞噬体。
Intracellular survival of C. psittaci is in part dependent on the ability of the organism to thwart phagolysosome formation. Circumvention of phagolysosome fusion could be localized to chlamydia-laden vacuoles or generalized to all phagososomes in the host cell. To determine which of these modes is in operation, the ability of Chlamydia elementary and reticulate bodies to protect Saccharomyces cerevisiae from degradation in macrophage (mouse) phagolysosomes was examined via acridine orange and Giemsa staining. No statistically significant differnece was evident between the amount of fusion observed in coinfected macrophages and those infected with yeast cells alone. This was not dependent on some unique interaction between the Chlamydia and the yeast cells since viable count studies to determine the protection of a 2nd organism, Escherichia coli, also failed to show significantly different amounts of inactivation of the bacteria by macrophages in the presence of C. psittaci. Thus the inhibition of phagolysosome fusion is localized to Chlaymdia-laden phagosomes.