Requirement of the fixed end for spontaneous beating in flagella
Requirement of the fixed end for spontaneous beating in flagella
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DOI：
10.1242/jeb.02131
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发表时间：
2006-04-01
影响因子：
2.8
通讯作者：
Okuno, M
中科院分区：
文献类型：
--
作者：
Fujimura, M;Okuno, M
众所周知，鞭毛的任何部分都有弯曲的能力。然而，鞭毛如何自发地产生连续的弯曲波还不清楚。一些显微操作实验表明鞭毛的基部是必需的。相比之下，在计算机模拟工作中，如果微管在一端捆绑在一起，则可以产生自发弯曲波。我们推测，鞭毛的基底结构只有在外部的双态微管紧密结合在一起而不滑动的情况下才能作为一个束缚端。我们开发了一种新的技术，在脱膜和重新激活的鞭毛上的任何位置引入局部抑制。在鞭毛上任何位置施加局部抑制时，鞭毛仍能保持自发跳动。此外，在鞭毛上引入人工固定区域后，鞭毛在没有基体的情况下发生了自发跳动。我们的结论是轴突，一束微管，需要固定端自发跳动。
It is well known that any part of a flagellum has the ability to bend. However, it is not clearly understood how flagella generate successive bending waves spontaneously. Some micromanipulation experiments have suggested that the base of the flagellum is required. By contrast, spontaneous bending waves could be generated in computer simulation work if the microtubules were tied together at one end. We hypothesized that the basal structure of flagella can only act as a tied end when the outer doublet microtubules are tightly bound together so as not to slide. We developed a new technique for introducing local inhibition at any position on the demembranated and reactivated flagellum. The flagellum maintained spontaneous beating when the local inhibition was introduced at any position on it. In addition, spontaneous beating occurred without the basal body when an artificial fixed region was introduced to the flagellum. We conclude that the axoneme, a bundle of microtubules, requires the fixed end for spontaneous beating.