Live births after autologous transplant of cryopreserved mouse ovaries
Live births after autologous transplant of cryopreserved mouse ovaries
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DOI：
10.1093/humrep/12.1.101
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发表时间：
1997-01-01
影响因子：
6.1
通讯作者：
Critser, JK
中科院分区：
文献类型：
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作者：
Gunasena, KT;Villines, PM;Critser, JK
本研究对小鼠自体卵巢移植后冷冻前后的生育力进行了研究。雌性小鼠随机分为假手术组(n=14)、卵巢切除组(n=11)、新鲜卵巢移植组(n=12)和冷冻保存组(n=11)。卵巢在1.4M二甲基亚砜(DMSO)中以0.5℃/min的速度冷冻至-55℃(冰核温度为-7℃)，在液氮中浸泡，然后在室温下解冻，在假手术后7天或新鲜或冷冻卵巢移植后15天观察发情周期。去卵巢动物没有表现出发情周期，但没有交配，也没有妊娠。所有的假手术、新鲜移植和8/11(73%)冷冻移植动物都记录了活产，新鲜移植组(4.32+/-0.44)和冷冻保存(4.71+/-0.57)移植组的总体平均+/-SEM窝产仔数较小(P<；0.05)高于假手术组(12.54+/-0.44)，但大小差异不显著(P>；0.05)。用4/4假手术、1/10新鲜和1/9冷冻卵巢移植动物的4窝活体幼鼠交配至少4次，假手术和移植动物幼鼠的产仔数无显著差异。小鼠卵巢自体移植后，冷冻前后后代形态正常，受精率高。
The fertility of mice after autologous transplantation of ovaries, before or after cryopreservation, was investigated in this study. Female mice were randomly assigned to either sham-operated (n = 14), ovariectomized (n = 11), fresh (n = 12) or cryopreserved (n = 11) ovarian transplant groups, Ovaries were cryopreserved in 1.4 M dimethyl sulphoxide (DMSO) by cooling to -55 degrees C at 0.5 degrees C/min (ice nucleation at -7 degrees C), plunged in liquid nitrogen and then thawed at room temperature, Oestrous cyclicity was observed 7 days after sham operation or 15 days after fresh or cryopreserved ovarian transplant, Ovariectomized animals did not demonstrate oestrous cyclicity but were mated, and no pregnancies resulted, Live births were recorded from all sham-operated, all fresh transplant, and 8/11 (73%) cryopreserved transplant animals, Overall mean +/- SEM litter sizes from fresh (4.32 +/- 0.44) and cryopreserved (4.71 +/- 0.57) transplant groups were smaller (P < 0.05) than those of sham-operated animals (12.54 +/- 0.44), although the sizes were not significantly different (P > 0.05) from each other. Animals were mated at least four times, with four litters of live pups from 4/4 sham-operated, 1/10 fresh and 1/9 cryopreserved ovarian transplant animals, Litter sizes from pups of sham-operated and transplant animals were not significantly different from each other, Following autologous transplantation of mouse ovaries, before or after cryopreservation, offspring appeared normal, with high rates of fertility.