Lipid Production by Culturing Oleaginous Yeast and Algae with Food Waste and Municipal Wastewater in an Integrated Process
Lipid Production by Culturing Oleaginous Yeast and Algae with Food Waste and Municipal Wastewater in an Integrated Process
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DOI：
10.1007/s12010-011-9263-6
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发表时间：
2011-09-01
影响因子：
3
通讯作者：
Chen, Shulin
中科院分区：
文献类型：
--
作者：
Chi, Zhanyou;Zheng, Yubin;Chen, Shulin
餐厨垃圾和城市废水是微生物脂质生物燃料生产的有前景的原料，相应的生产工艺有待开发。在这项研究中，测试了不同的产油酵母菌株在水解食物垃圾中的生长情况，在此条件下弯曲隐球菌、解脂耶氏酵母和粘红酵母的生长水平与作为对照的葡萄糖培养物中的生长水平相同。进一步测试了这些菌株在城市初级废水中的生长情况。在由初级废水制成的培养基中，无论添加或不添加葡萄糖，C. curvatus 和 R. glutinis 的产量均高于 Y. lipolytica。最后，测试了在食物垃圾和城市废水制成的培养基中生长 C. curvatus 和 R. glutinis 的工艺，并用酵母培养物和光养藻类培养物进一步处理这些工艺的废水；在第二步酵母培养物中进一步产生 1.1 g/L C. curvatus 和 1.5 g/L R. glutinis 生物量，以及在光养培养物中产生 1.53 和 0.58 g/L Chlorella sorokiniana 生物量。最终出水残留氮浓度分别为33 mg/L和34 mg/L，残留磷浓度分别为1.5和0.6 mg/L。产生的生物质中的脂质含量为18.7%至28.6%。
Food waste and municipal wastewater are promising feedstocks for microbial lipid biofuel production, and corresponding production process is to be developed. In this study, different oleaginous yeast strains were tested to grow in hydrolyzed food waste, and growths of Cryptococcus curvatus, Yarrowia lipolytica, and Rhodotorula glutinis in this condition were at same level as in glucose culture as control. These strains were further tested to grow in municipal primary wastewater. C. curvatus and R. glutinis had higher production than Y. lipolytica in media made from primary wastewater, both with and without glucose supplemented. Finally, a process was tested to grow C. curvatus and R. glutinis in media made from food waste and municipal wastewater, and the effluents from these processes were further treated with yeast culture and phototrophic algae culture; 1.1 g/L C. curvatus and 1.5 g/L R. glutinis biomass were further produced in second-step yeast cultures, as well as 1.53 and 0.58 g/L Chlorella sorokiniana biomass in phototrophic cultures. The residual nitrogen concentrations in final effluents were 33 mg/L and 34 mg/L, respectively, and the residual phosphorus concentrations were 1.5 and 0.6 mg/L, respectively. The lipid contents in the produced biomass were from 18.7% to 28.6%.