Lysobacter capsici AZ78 produces cyclo(L-Pro-L-Tyr), a 2,5-diketopiperazine with toxic activity against sporangia of Phytophthora infestans and Plasmopara viticola
Lysobacter capsici AZ78 produces cyclo(L-Pro-L-Tyr), a 2,5-diketopiperazine with toxic activity against sporangia of Phytophthora infestans and Plasmopara viticola
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DOI：
10.1111/jam.12611
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发表时间：
2014-10-01
影响因子：
4
通讯作者：
Pertot, I.
中科院分区：
文献类型：
--
作者：
Puopolo, G.;Cimmino, A.;Pertot, I.
目的：研究辣椒溶杆菌（Lysobacter capsici）AZ 78体外产生的小分子化合物及其对植物病原卵菌孢子囊的毒力。辣椒AZ 78显著抑制植物病原卵菌的生长。因此，L.辣椒AZ 78在葡萄藤和番茄植株上分别降低了单轴霉属（Plasmopara（Pl.）Viticola和Phytophthora infestans。对L.通过光谱学（主要是1D和2D H-1 NMR和C-13 NMR和ESI MS光谱）和光学方法对辣椒AZ 78进行鉴定，鉴定了在体外和番茄叶上抑制致病疫霉孢子囊发育的2，5-二酮哌嗪环（L-Pro-L-Tyr）。此外，在L.结论：辣椒溶杆菌（Lysobacter capsici）AZ 78在体外产生环（L-Pro-L-Tyr），其对致病疫霉（P. infestans）和辣椒疫霉（Pl.体外葡萄球菌。此外，这种低分子量化合物在施用于番茄叶片上时防止晚疫病病变的发生。辣椒AZ 78细胞或其自身培养物有效地控制致病疫霉和Pl. Viticola。L-脯氨酸-L-酪氨酸环（L-Pro-L-Tyr）是由L.辣椒AZ 78对这两种卵菌的孢子囊都有毒性。这些数据增强了溶杆菌属成员用于控制植物病原性卵菌的潜力，并为开发基于环（L-Pro-L-Tyr）的新型低影响杀真菌剂提供了基础。
Aims: To investigate low molecular weight compounds produced in vitro by Lysobacter capsici AZ78 and their toxic activity against sporangia of plant pathogenic oomycetes.Methods and Results: Assays carried out in vitro showed that L. capsici AZ78 drastically inhibits the growth of plant pathogenic oomycetes. Accordingly, the preventive application of culture filtrates of L. capsici AZ78 on grapevine and tomato plants reduced the infections, respectively, caused by Plasmopara (Pl.) viticola and Phytophthora infestans. The subsequent chemical analysis of the culture filtrates of L. capsici AZ78 by spectroscopic (essentially 1D and 2D H-1 NMR and C-13 NMR and ESI MS spectra) and optical methods led to the identification of the 2,5-diketopiperazine cyclo(L-Pro-L-Tyr) that inhibited the development of P. infestans sporangia in vitro and on tomato leaves. Furthermore, a genomic region with high sequence identity with genes coding for a hybrid polyketide synthase and nonribosomal peptide synthetase was detected in L. capsici AZ78.Conclusions: Lysobacter capsici AZ78 produces cyclo(L-Pro-L-Tyr) in vitro that was effective in killing the sporangia of P. infestans and Pl. viticola in vitro. Moreover, this low molecular weight compound prevents the occurrence of late blight lesions when applied on tomato leaves.Significance and Impact of the Study: The application of L. capsici AZ78 cells or its own culture filtrates effectively controls both P. infestans and Pl. viticola. Cyclo(L-Pro-L-Tyr) produced by L. capsici AZ78 is toxic against sporangia of both these oomycetes. These data enforce the potential in the use of Lysobacter members for the control of plant pathogenic oomycetes and provide the basis for the development of new low-impact fungicides based on cyclo(L-Pro-L-Tyr).