A unique ribonucleoprotein complex assembles preferentially on ecdysone-responsive sites in Drosophila melanogaster.
A unique ribonucleoprotein complex assembles preferentially on ecdysone-responsive sites in Drosophila melanogaster.
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一种独特的核糖核蛋白复合物优先在果蝇的蜕皮激素反应位点上组装。
DOI：
10.1128/mcb.13.9.5323-5330.1993
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发表时间：
1993
影响因子：
5.3
通讯作者：
Beyer,AL
中科院分区：
文献类型：
--
作者：
Amero,SA;Matunis,MJ;Matunis,EL;Hockensmith,JW;Raychaudhuri,G;Beyer,AL
蜕皮激素喷流上的蛋白质（PEP）优先与果蝇多雌期染色体上的活性蜕皮激素诱导喷流相关，并含有转录因子和RNA结合蛋白的特征序列基序（S. A. Amero，S. C. R.埃尔金和A. L. Beyer，Genes Dev. 5：188-200，1991）。PEP在体内与RNA相关，如PEP特异性染色体原位免疫染色对RNA酶消化的敏感性和PEP在果蝇细胞提取物中与异质核核糖核蛋白（hnRNP）复合物的免疫纯化所证明的。如通过连续免疫染色所揭示的，PEP被发现在由HRB（异质核RNA结合）蛋白结合的染色体位点的子集上，HRB蛋白是基本的果蝇RNPs。这些观察结果使我们建议，一个独特的，PEP-含有hnRNP复合物组装优先的蜕皮激素调节基因inDrosophila melanogasterprobably加快转录和/或处理这些成绩单的成绩单。
The protein on ecdysone puffs (PEP) is associated preferentially with active ecdysone-inducible puffs onDrosophilapolytene chromosomes and contains sequence motifs characteristic of transcription factors and RNA-binding proteins (S. A. Amero, S. C. R. Elgin, and A. L. Beyer, Genes Dev. 5:188-200, 1991). PEP is associated with RNA in vivo, as demonstrated here by the sensitivity of PEP-specific chromosomal immunostaining in situ to RNase digestion and by the immunopurification of PEP inDrosophilacell extract with heterogeneous nuclear ribonucleoprotein (hnRNP) complexes. As revealed by sequential immunostaining, PEP is found on a subset of chromosomal sites bound by the HRB (heterogeneous nuclear RNA-binding) proteins, which are basicDrosophilahnRNPs. These observations lead us to suggest that a unique, PEP-containing hnRNP complex assembles preferentially on the transcripts of ecdysone-regulated genes inDrosophila melanogasterpresumably to expedite the transcription and/or processing of these transcripts.