Zonal differences in DNA synthesis and in transglutaminase activity between perivenous versus periportal regions of regenerating rat liver.
Zonal differences in DNA synthesis and in transglutaminase activity between perivenous versus periportal regions of regenerating rat liver.
复制标题
再生大鼠肝脏的静脉周围区域与门静脉周围区域之间 DNA 合成和转谷氨酰胺酶活性的区域差异。
DOI：
--
复制
发表时间：
2004
影响因子：
2
通讯作者：
Y. Ohkubo
中科院分区：
文献类型：
--
作者：
Y. Ohtake;A. Maruko;S. Kojima;T. Ono;Tomomi Nagashima;M. Fukumoto;Satoko Suyama;S. Abe;N. Sato;Y. Ohkubo
我们调查了区域内的DNA合成和转氨酶（TGase）活性之间的静脉周围与门静脉周围区域的再生大鼠肝脏的差异的关系。使用毛地黄皂苷/胶原酶灌注技术，肝细胞亚群分离从每个区域在不同的时间点后，部分肝切除术。测定各亚群的DNA合成量以及TGase mRNA水平和活性。虽然在两个亚群中观察到增加的DNA合成在部分肝切除术后24 h达到峰值，门静脉周围肝细胞（PPH）的DNA合成量是静脉周围肝细胞（PVH）的两倍。在PVH中，TGase活性在部分肝切除后24 h达到峰值，其mRNA表达在12 h之前增加，而在PPH中24 h的TGase活性是PVH中的一半。由于已知TGase具有生长抑制活性，我们的数据表明，部分肝切除术后24小时PVH中相对较高的TGase活性可能与该区域相对较低的DNA合成相关。
We investigated a relationship within zonal differences in DNA synthesis and in transglutaminase (TGase) activity between perivenous versus periportal regions of regenerating rat liver. Using the digitonin/collagenase perfusion technique, hepatocyte subpopulations were isolated from each region at various time points after partial hepatectomy. The amounts of DNA synthesis as well as the levels of TGase mRNA and activity in each subpopulation were measured. Although increased DNA synthesis was observed in both subpopulations with a peak at 24 h after partial hepatectomy, the amount of DNA synthesis in periportal hepatocytes (PPH) was twice as much as that in perivenous hepatocytes (PVH). In PVH, TGase activity peaked at 24 h after partial hepatectomy with a preceding increase in its mRNA expression at 12 h, whereas TGase activity in PPH at 24 h was one-half of that in PVH. As TGase is known to have a growth-arresting activity, our data indicate that relatively higher TGase activity in PVH at 24 h after partial hepatectomy might correlate with relatively lower DNA synthesis in this region compared to periportal region.
血小板活化因子和血小板活化因子拮抗剂调节体外免疫反应。
DOI：
10.1016/0162-3109(88)90038-0
发表时间：
1988
期刊：
Immunopharmacology
影响因子：
--
作者：
Gebhardt,BM;Braquet,P;Bazan,H;Bazan,N
通讯作者：
Bazan,N