A novel sandwich-type electrochemical immunosensor for PSA detection based on PtCu bimetallic hybrid (2D/2D) rGO/g-C3N4
A novel sandwich-type electrochemical immunosensor for PSA detection based on PtCu bimetallic hybrid (2D/2D) rGO/g-C3N4
复制标题
基于 PtCu 双金属杂化 (2D/2D) rGO/g-C3N4 的新型夹心型 PSA 电化学免疫传感器
DOI：
10.1016/j.bios.2016.12.070
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发表时间：
2017-05-15
影响因子：
12.6
通讯作者：
Wei, Qin
中科院分区：
文献类型：
--
作者：
Feng, Jinhui;Li, Yueyun;Wei, Qin
本文设计了一种灵敏的三明治型电化学免疫传感器，用于安培i-t法定量检测前列腺特异性抗原（PSA）。将负载在硫氨酸功能化氧化石墨烯（Au@Th/GO）上的Au作为固定一抗（Ab(2)）的平台，并加速电极界面上的电子转移。将PtCu双金属杂化物负载在具有大表面积和生物相容性的2D/2D还原氧化石墨烯/氮化石墨碳（PtCu@rGO/g-C3N4）上，作为结合二抗（Ab(2)）和放大信号的标记物，以提高所设计的免疫传感器对过氧化氢（H2O2）还原的灵敏度。在最佳条件下，所设计的免疫传感器对PSA的检测下限为16.6 fg/mL (S/N=3)，在50 ~ 40 ng/mL范围内呈线性变化。此外，所设计的免疫传感器具有良好的选择性、重复性和稳定性。结果表明，该方法在临床肿瘤标志物监测中具有较好的应用前景。
In this work, a sensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of prostate-specific antigen (PSA) by amperometric i-t. The Au loaded on thionine functionalized graphene oxide (Au@Th/GO) was used as a platform to immobilize primary antibodies (Ab(2)) and accelerate the electron transfer on the electrode interface. PtCu bimetallic hybrid were loaded on 2D/2D reduced graphene oxide/graphitic carbon nitride (PtCu@rGO/g-C3N4) with large surface area and biocompatibility, which were employed as labels for combining secondary antibodies (Ab(2)) and amplifying signals to improve the sensitivity of the designed immunosensor which attributes to its good activity, for the reduction of hydrogen peroxide (H2O2). Under optimal conditions, the designed immunosensor exhibited a linear concentration range from 50 fg/mL to 40 ng/mL, with a low detection limit of 16.6 fg/mL (S/N=3) for PSA. Additionally, the designed immunosensor showed acceptable selectivity, reproducibility and stability. The satisfactory results in analyze human serum samples indicated potential application promising in clinical monitoring of tumor markers.