FAB-MS characterization of sialyl Lewisx determinants on polylactosamine chains of human airway mucins secreted by patients suffering from cystic fibrosis or chronic bronchitis
FAB-MS characterization of sialyl Lewisx determinants on polylactosamine chains of human airway mucins secreted by patients suffering from cystic fibrosis or chronic bronchitis
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DOI：
10.1023/a:1020871322769
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发表时间：
2001-09-01
影响因子：
3
通讯作者：
Dell, A
中科院分区：
文献类型：
--
作者：
Morelle, W;Sutton-Smith, M;Dell, A
虽然大量的结构数据存在于囊性纤维化（CF）和慢性支气管炎（CB）患者的支气管粘蛋白，很少有人知道的聚-N-乙酰乳糖胺天线上进行的终端结构。这些结构是令人感兴趣的，因为它们是细菌粘附素和其他凝集素的潜在配体。在这项研究中，我们已经使用快原子轰击质谱（FAB-MS）检查的末端序列释放的内切β-半乳糖苷酶从O-聚糖获得还原消除支气管粘蛋白纯化的8 CF和8 CB患者的痰液。我们的数据表明，虽然CF和CB粘蛋白的聚乳糖胺触角有几个共同的末端序列，但它们的唾液酸刘易斯（x）（NeuAcalpha 2 - 3Galbeta 1 -4[Fucalpha 1 -3] GlcNAc beta1-）含量显著不同。因此，来自CF患者的所有检查的粘蛋白在它们的聚乳糖胺触角上携带唾液酸刘易斯（x），而这种类型的表位仅存在于检查的8种CB粘蛋白中的3种上，特别是在被铜绿假单胞菌严重感染的一名CB患者的气道中，所有CF患者的气道也是如此。这表明，在气道粘蛋白中，唾液酸刘易斯（x）在多聚乳糖胺触角上的表达可能与炎症和感染的关系比CF缺陷的直接影响更密切。
Although a large body of structural data exists for bronchial mucins from cystic fibrosis (CF) and chronic bronchitis (CB) patients, little is known about terminal structures carried on poly-N-acetyllactosamine antennae. Such structures are of interest because they are potential ligands for bacterial adhesins and other lectins. In this study, we have used fast atom bombardment mass spectrometry (FAB-MS) to examine terminal sequences released by endo-beta-galactosidase from O-glycans obtained by reductive elimination of bronchial mucins purified from the sputum of 8 CF and 8 CB patients. Our data show that, although the polylactosamine antennae of CF and CB mucins have several terminal sequences in common, they differ significantly in their sialyl Lewis(x) (NeuAcalpha2-3Galbeta1-4[Fucalpha1-3] GlcNAcbeta1-) content. Thus all examined mucins from CF patients carry sialyl Lewis(x) on their polylactosamine antennae, whereas this type of epitope is present on only three out of the eight CB mucins examined, notably in the airways of one CB patient which were heavily infected by Pseudomonas aeruginosa as are the airways of all the CF patients. This suggests that, in airway mucins, the expression of sialyl Lewis(x) on polylactosamine antennae is probably more related to inflammation and infection than to a direct effect of the CF defect.