A monoclonal anti-peptide antibody recognizes the adrenocorticotropic receptor.
A monoclonal anti-peptide antibody recognizes the adrenocorticotropic receptor.
复制标题
单克隆抗肽抗体可识别促肾上腺皮质激素受体。
DOI：
10.1016/0006-291x(90)91131-b
复制
发表时间：
1990
影响因子：
3.1
通讯作者：
Bost,KL
中科院分区：
文献类型：
--
作者：
Clarke,BL;Bost,KL
我们已经研制出一种能特异性识别大鼠肾上腺细胞上促肾上腺皮质激素受体的单抗。该免疫原由与编码ACTH1-24的mRNA互补的RNA序列设计而成。这种互补的多肽被称为HTCA，已被证明能与ACTH特异结合，并被提议模拟激素受体的ACTH结合部位。抗HTCA的单抗识别HTCA的一个限制性结构域，结合Y-1肾上腺细胞的KD值为1.8 nM，并阻断了125I-ACTH与大鼠肾上腺细胞的结合。这些发现表明，抗HTCA与ACTH竞争结合ACTH受体。
We have produced a monoclonal antibody that specifically recognizes the adrenocorticotropic receptor on rat adrenal cells. The immunogen was designed from an RNA sequence complementary to the mRNA coding for ACTH1–24. This complementary peptide, termed HTCA, has been shown to specifically bind ACTH and was proposed to mimic the ACTH binding site of the hormone receptor. The monoclonal anti-HTCA antibody recognized a restricted domain of the HTCA peptide, bound to Y-1 adrenal cells with a KDof 1.8 nM, and blocked the binding of125I-ACTH to rat adrenal cells. These findings show that anti-HTCA competes with ACTH for binding to the ACTH receptor.