Three-dimensional architecture of the bacteriophage φ29 packaged genome and elucidation of its packaging process
Three-dimensional architecture of the bacteriophage φ29 packaged genome and elucidation of its packaging process
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DOI：
10.1016/j.virol.2007.07.035
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发表时间：
2008-02-20
期刊：
影响因子：
3.7
通讯作者：
Downing, Kenneth H.
中科院分区：
文献类型：
--
作者：
Comolli, Luis R.;Spakowitz, Andrew J.;Downing, Kenneth H.
这项工作的目的是了解dsDNA噬菌体中DNA包装过程的精确分子机制。低温电子显微镜被用来直接显示衣壳内DNA的结构，从而测量基本的物理参数，如链间距、局部曲率和有序度。我们获得了噬菌体的冷冻-EM图像，这些噬菌体包装了基因组的特定片段，以及部分完成包装过程的颗粒。对中间和最后阶段观察到的结构进行的结果比较表明，没有唯一的、确定的DNA包装途径。包装过程的蒙特卡罗模拟提供了对所涉及的力和所产生的结构的洞察。(C)2007 Elsevier Inc.保留所有权利。
The goal of the work reported here is to understand the precise molecular mechanism of the process of DNA packaging in dsDNA bacteriophages. Cryo-EM was used to directly visualize the architecture of the DNA inside the capsid and thus to measure fundamental physical parameters such as inter-strand distances, local curvatures, and the degree of order. We obtained cryo-EM images of bacteriophage that had packaged defined fragments of the genome as well as particles that had partially completed the packaging process. The resulting comparison of structures observed at intermediate and final stages shows that there is no unique, deterministic DNA packaging pathway. Monte Carlo simulations of the packaging process provide insights on the forces involved and the resultant structures. (C) 2007 Elsevier Inc. All rights reserved.