Major histocompatibility complex class II alleles in Kazak and Han populations in the Silk Route of northwestern China
Major histocompatibility complex class II alleles in Kazak and Han populations in the Silk Route of northwestern China
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DOI：
10.1111/j.1399-0039.1997.tb02909.x
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发表时间：
1997-11-01
期刊：
影响因子：
--
通讯作者：
Inoko, H
中科院分区：
文献类型：
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作者：
Mizuki, N;Ohno, S;Inoko, H
采用聚合酶链式反应-限制性片段长度多态性方法，对新疆维吾尔族自治区乌鲁木齐市中国最西北的哈萨克族人群和同一地区的汉族人群进行了DRB1、DQA1、DQB1和DPB1四个基因座的遗传多态性研究。42名哈萨克族和59名汉族无血缘关系志愿者参加了本研究。在51个DRB1等位基因中，共检出29个等位基因，其中哈萨克族DRB1*0301(13.1%)和DRB1*07(10.7%)，西北汉族以DRB1*0901(11.9%)、DRB1*1501(11.0%)和DRB1*07(11.0%)为主。在检测到的8个DQA1等位基因中，哈萨克族的DQA1*0501(29.8%)和DQA1*0301(23.8%)是最常见的等位基因，西北汉族的DQA1*0301(28.8%)和DQA1*0102(19.5%)是最常见的等位基因。在18个DQB1等位基因中，共检出14个等位基因，其中DQB1*0201和DQB1*0301在哈萨克族(分别为23.8%和21.4%)和西北汉族(分别为18.6%和16.9%)中均非常常见。在检测的37个DPB1等位基因中，有14个被检测到。其中，哈萨克族DPB1*0401(21.4%)、DPB1*0501(20.2%)、DPB1*0402(19.0%)、DPB1*0201(16.7%)等位基因频率显著增加，西北汉族DPB1*0501(38.1%)、DPB1*0201(16.1%)等位基因频率显著升高。哈萨克族DRB1*0301-DQA1*0501-DQB1*0201(13.1%)和DRB1*0701-DQA1*0201-DQB1*0201(8.3%)；西北部汉族DRB1*0901-DQA1*0301-DQB1*0303(11.9%)和DRB1*0701-DQA1*0201-DQB1*0201(10.2%)。根据中国北方汉族、南方汉族、满族和日本人等12个代表性人群的DRB1、DQA1、DQB1和DPB1基因的等位基因频率，采用邻接法(NJ)构建的树状图表明哈萨克族和西北汉族距离最近，而哈萨克族与亚洲民族的距离比西北汉族略远。
Genetic polymorphism of the HLA class II loci including the DRB1, DQA1, DQB1 and DPB1 genes was investigated by the polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) method in a Kazak population inhabiting the most northwestern part of China, Urumqi in the Xinjiang Uygur Zizhiqu as well as in a Han population in the same area. Forty-two Kazak and 59 Han unrelated volunteers were enrolled in this study. Among 51 DRB1 alleles tested, 29 alleles were detected, and DRB1*0301 (13.1%) and DRB1*07 (10.7%) in Kazak and DRB1*0901 (11.9%), DRB1*1501 (11.0%) and DRB1*07 (11.0%) in northwestern Han were highly predominant. In 8 DQA1 alleles detected, DQA1*0501 (29.8%) and DQA1*0301 (23.8%) in Kazak, and DQA1*0301 (28.8%) and DQA1*0102 (19.5%) in northwestern Han were the most and the second most common alleles, respectively. Of 18 DQB1 alleles tested, 14 were observed, among which DQB1*0201 and DQB1*0301 were very frequent both in Kazak (23.8% and 21.4%, respectively) and northwestern Han (18.6% and 16.9%, respectively) populations. Of 37 DPB1 alleles tested, 14 were detected. Among them, the frequencies of DPB1*0401 (21.4%), DPB1*0501 (20.2%), DPB1*0402 (19.0%) and DPB1*0201 (16.7%) in Kazak, and those of DPB1*0501 (38.1%) and DPB1*0201 (16.1%) in northwestern Han were highly increased. Several three-locus haplotypes were recognized to predominate significantly, namely DRB1*0301-DQA1*0501-DQB1*0201 (13.1%) and DRB1*0701-DQA1*0201-DQBl*0201 (8.3%) in Kazak; and DRB1*0901-DQA1*0301-DQB1*0303 (11.9%) and DRB1*0701-DQA1*0201-DQB1*0201 (10.2%) in northwestern Han. The dendrogram constructed by the neighbor-joining (NJ) method based on the allele frequencies of the DRB1, DQA1, DQB1 and DPB1 genes of 12 representative populations all over the world including northern Han, southern Han, Manchu and Japanese suggested that Kazak and northwestern Han were the closest to each other, but Kazak was a little farther from the Asian ethnic groups than northwestern Han.