Changes in Brain Protease Activity in Aging
Changes in Brain Protease Activity in Aging
复制标题
衰老过程中脑蛋白酶活性的变化
DOI：
10.1046/j.1471-4159.1996.67052019.x
复制
发表时间：
1996
影响因子：
4.7
通讯作者：
A. Lajtha
中科院分区：
文献类型：
--
作者：
M. Benuck;M. Banay‐Schwartz;Teresita DeGuzman;A. Lajtha
摘要：我们测量了蛋白酶活性随衰老的变化，检测了组织蛋白酶 D 和钙蛋白酶 II 的活性以及细胞骨架蛋白的降解率，并制备了年轻和老年大脑中的酶和底物。添加到年轻和老化底物中的钙蛋白酶制剂以酪蛋白作为底物标准化，以便测量与年龄相关的钙蛋白酶特异性和底物敏感性的变化。在底物敏感性和酶活性方面观察到一些与年龄相关的差异。就底物而言，年轻动物的神经丝蛋白比年老动物的神经丝蛋白更容易受到钙蛋白酶作用的影响。就酶活性而言，老年大脑中的钙蛋白酶裂解神经丝蛋白的速度比年轻大脑中的钙蛋白酶更快。对于神经丝，当老化组织的酶与年轻组织的底物一起孵育时，通常会发生最快速的分解。与老年大鼠的神经丝底物一起孵育的老年大鼠的肾酶比与年轻大鼠的底物一起孵育的年轻肾的酶导致更快的分解。微管蛋白分解的年龄依赖性与神经丝分解的年龄依赖性有些不同。最快速的分解通常发生在使用幼苗酶和幼苗微管蛋白时。神经丝蛋白或微管蛋白与组织蛋白酶 D 的孵育并未显示出任何与衰老相关的差异。这些研究表明，先前在衰老过程中观察到的酶活性的增加还可能包括酶特性（底物特异性）和/或其内源性底物特性（分解敏感性）的变化。
Abstract: We measured changes in protease activity with aging, conducting assays of cathepsin D and calpain II activities and the rate of degradation of cytoskeletal proteins, preparing the enzymes and substrates from young and aged brains. Calpain preparations added to the young and to the aged substrates were standardized with casein as substrate so that age‐related changes in calpain specificity and substrate susceptibility were measured. Several age‐related differences were observed in substrate susceptibility and in enzyme activity. With respect to substrate, the neurofilament protein from young animals was somewhat more susceptible to calpain action than that from older animals. With respect to enzyme activity, calpain from aged brain cleaved neurofilament protein at a faster rate than did calpain from young. With neurofilaments, the most rapid breakdown usually occurred when enzyme from aged tissue was incubated with substrate from young. Kidney enzyme of aged rats incubated with neurofilament substrate of aged rats resulted in a more rapid breakdown than enzyme of young kidney incubated with substrate of young. The age dependence of tubulin breakdown was somewhat different from that of neurofilament breakdown. The most rapid breakdown usually occurred when using enzyme from young with tubulin from young. Incubation of neurofilament protein or tubulin with cathepsin D did not reveal any differences with aging. These studies suggest that an increase in enzyme activity observed previously during aging may also include changes in the properties of the enzyme (substrate specificity) and/or in the properties of their endogenous substrates (susceptibility to breakdown).
登录
查看更多内容
降解神经丝蛋白的脑钙激活蛋白酶的表征。
影响因子：
2.9
作者：
Zimmerman,UJ;Schlaepfer,WW
通讯作者：
Schlaepfer,WW
白内障和钙蛋白酶诱导的 β-晶状体蛋白不溶解的加速发生在大鼠晶状体正常成熟过程中。
影响因子：
4.4
作者：
David,LL;Azuma,M;Shearer,TR
通讯作者：
Shearer,TR
DOI：
10.1073/pnas.90.7.2628
发表时间：
1993-04-01
影响因子：
11.1
作者：
SAITO, KI;ELCE, JS;NIXON, RA
通讯作者：
NIXON, RA
脑中可溶性钙依赖性蛋白水解活性的比较研究。
DOI：
10.1002/neu.480170103
发表时间：
1986
期刊：
Journal of neurobiology
影响因子：
--
作者：
Baudry,M;Simonson,L;Dubrin,R;Lynch,G
通讯作者：
Lynch,G