Periplocoside A, a pregnane glycoside from Periploca sepium Bge, prevents concanavalin A-induced mice hepatitis through inhibiting NKT-derived inflammatory cytokine productions.
Periplocoside A, a pregnane glycoside from Periploca sepium Bge, prevents concanavalin A-induced mice hepatitis through inhibiting NKT-derived inflammatory cytokine productions.
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DOI：
10.1016/j.intimp.2008.05.001
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发表时间：
2008-09
影响因子：
5.6
通讯作者：
Jin Wan;Yi-na Zhu;Jiaquan Feng;Hai-jun Chen;Ru-jun Zhang;Jia Ni;Zhen-hua Chen;Li-fei Hou;Quanhong Liu;Jing Zhang;Li Yang-;Wei Tang;Yi-fu Yang;F. Nan;Weimin Zhao;Jian-Ping Zuo
中科院分区：
文献类型：
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作者：
Jin Wan;Yi-na Zhu;Jiaquan Feng;Hai-jun Chen;Ru-jun Zhang;Jia Ni;Zhen-hua Chen;Li-fei Hou;Quanhong Liu;Jing Zhang;Li Yang-;Wei Tang;Yi-fu Yang;F. Nan;Weimin Zhao;Jian-Ping Zuo
黄芩是一种传统的中草药，在中国被广泛用于治疗类风湿性关节炎。Periplocoside A （PSA）是一种妊娠糖苷类化合物，是从蛇皮草中分离得到的天然产物。我们检测了PSA对大麻碱A （ConA）诱导肝炎的保护作用。PSA预处理可显著改善cona诱导的肝损伤，其特征是降低血清丙氨酸转氨酶（ALT）、致病性细胞因子白介素(IL)-4和干扰素(IFN)-γ水平，阻止肝坏死，从而提高生存率。在体外，PSA抑制α-半乳糖神经酰胺（α-GalCer）或抗cd3激活的自然杀伤T （NKT）细胞产生IL-4和IFN-γ。酶联免疫吸附试验（ELISA）和逆转录聚合酶链反应（RT-PCR）结果显示，PSA抑制IL-4转录和IFN-γ翻译。由此可见，PSA对cona诱导的肝炎具有显著的预防作用，其作用机制与抑制nkt源性炎症细胞因子的产生密切相关。这些发现提示PSA具有治疗人类自身免疫性肝炎的治疗潜力。
Periploca sepium Bge, a traditional Chinese herb medicine, is widely used for treating rheumatoid arthritis in china. Periplocoside A (PSA), a pregnane glycoside, is a new nature product compound isolated from P. sepium Bge. We examined the protective effects of PSA, on concanavaline A (ConA)-induced hepatitis. Pretreatment with PSA dramatically ameliorated ConA-induced liver injury, which was characterized by reducing serum alanine transaminase (ALT), pathogenic cytokines of interleukin (IL)-4 and interferon (IFN)-γ levels, impeding the liver necrosis, and thus elevating the survival rate. In vitro, PSA inhibited IL-4 and IFN-γ productions of α-galactosylceramide (α-GalCer) or anti-CD3-activated Natural killer T (NKT) cells. Enzyme Linked Immunosorbent Assay (ELISA) and Reverse Transcription Polymerase Chain Reaction (RT-PCR) assays revealed PSA suppressed IL-4 transcription and IFN-γ translation. In conclusion, PSA had significantly preventative effect on ConA-induced hepatitis, which was closely associated with inhibition of NKT-derived inflammatory cytokine productions. These findings suggested that PSA has the therapeutic potential for treatment of human autoimmune-related hepatitis.