TEMPORAL EXPRESSION OF REGULATORY AND STRUCTURAL MUSCLE PROTEINS DURING MYOGENESIS OF SATELLITE CELLS ON ISOLATED ADULT-RAT FIBERS
TEMPORAL EXPRESSION OF REGULATORY AND STRUCTURAL MUSCLE PROTEINS DURING MYOGENESIS OF SATELLITE CELLS ON ISOLATED ADULT-RAT FIBERS
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DOI：
10.1006/dbio.1994.1226
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发表时间：
1994-08-01
影响因子：
2.7
通讯作者：
RIVERA, AJ
中科院分区：
文献类型：
--
作者：
YABLONKAREUVENI, Z;RIVERA, AJ
成年骨骼肌(卫星细胞)中的成肌前体细胞处于有丝分裂静止状态，但可以在包括肌肉损伤在内的各种压力下增殖。为了进一步了解成年成肌细胞，我们分析了从成年大鼠肌肉分离的完整纤维上卫星细胞的肌肉发生。在这种培养模式中，卫星细胞保持在纤维基底膜下的原位位置。在目前的研究中，通过培养纤维的免疫组织化学方法，确定了卫星细胞的增殖模式、成肌调节因子蛋白的表达以及分化特异的细胞骨架蛋白的表达。增殖细胞核抗原(增殖细胞核抗原)和MyoD阳性细胞的时间形态和细胞数量相似，表明MyoD在增殖中的卫星细胞中存在，但在静止的卫星细胞中不存在。随着增殖细胞核抗原和肌球蛋白表达的下降，出现了肌生成素、铜肌动蛋白(αSMactin)或发育肌节肌球蛋白(DEVmyosin)阳性的卫星细胞。然而，肌生成素和α-SMactin的表达是短暂的，而DEVmyosin的表达是持续的。此外，DEVmyosin+细胞的数量仅为mygenin+或αSMactin+细胞数量的一半，这可能表明只有50%的卫星细胞后代进入终末分化阶段。我们进一步确定，基本成纤维细胞生长因子可以调节增殖卫星细胞的数量，但不影响细胞周期进入、增殖、分化以及调控和结构蛋白的时间表达的总体时间表。因此，我们得出结论，当卫星细胞在其沿肌肉纤维的原始位置进行肌肉发生时，符合高度协调的程序。(C)1994年学术出版社。
Myogenic precursors in adult skeletal muscle (satellite cells) are mitotically quiescent but can proliferate in response to a variety of stresses including muscle injury. To gain further understanding of adult myoblasts, we analyzed myogenesis of satellite cells on intact fibers isolated from adult rat muscle. In this culture model, satellite cells are maintained in their in situ position underneath the fiber basement membrane. In the present study patterns of satellite cell proliferation, expression of myogenic regulatory factor proteins, and expression of differentiation-specific, cytoskeletal proteins were determined, via immunohistochemistry of cultured fibers. The temporal appearance and the numbers of cells positive for proliferating cell nuclear antigen (PCNA) or for MyoD were similar, suggesting that MyoD is present in detectable amounts in proliferating but not quiescent satellite cells. Satellite cells positive for myogenin, cu-smooth muscle actin (alpha SMactin), or developmental sarcomeric myosin (DEVmyosin) appeared following the decline in PCNA and MyoD expression. However, expression of myogenin and alpha SMactin was transient, while DEVmyosin expression was continuously maintained. Moreover, the number of DEVmyosin+ cells was only half of the number of myogenin+ or alpha SMactin+ cells-indicating, perhaps, that only 50% of the satellite cell descendants entered the phase of terminal differentiation. We further determined that the number of proliferating satellite cells can be modulated by basic FGF but the overall schedule of cell cycle entry, proliferation, differentiation, and temporal expression of regulatory and structural proteins was unaffected. We thus conclude that satellite cells conform to a highly coordinated program when undergoing myogenesis at their native position along the muscle fiber. (C) 1994 Academic Press, Inc.