A novel method for screening colorectal cancer by infrared spectroscopy of peripheral blood mononuclear cells and plasma
A novel method for screening colorectal cancer by infrared spectroscopy of peripheral blood mononuclear cells and plasma
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DOI：
10.1007/s00535-015-1095-7
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发表时间：
2016-03-01
影响因子：
6.3
通讯作者：
Wasserberg, Nir
中科院分区：
文献类型：
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作者：
Barlev, Eyal;Zelig, Udi;Wasserberg, Nir
背景结直肠癌（CRC）的早期发现可以降低死亡率和发病率。目前的筛查方法包括结肠镜检查和粪便检查，但一个简单的低成本血液检查将增加依从性。本初步研究评估的效用分析外周血单核细胞（PBMC）和血浆的整个生物分子概况，使用傅里叶变换红外（FTIR）光谱早期检测CRC.Methods血液样本前瞻性收集62名候选人的CRC筛查/诊断结肠镜检查或手术结肠肿瘤。通过Ficoll梯度分离PBMC和血浆，在硒化锌载玻片上干燥，并置于FTIR显微镜下。FTIR光谱分析的生物标志物和分类的主成分和判别分析。结果在外周血单个核细胞（p = 0.01）和血浆（p = 0.0001）光谱中观察到可作为CRC生物标志物的多个条带的显著变化。健康个体和良性息肉患者的血液成分存在微小但统计学显著差异。多因素分析显示，健康人群与结直肠癌患者的分布差异显著，良性息肉患者多分布在重叠区域内。留一法交叉验证评价方法的性能产生了0.77的受试者工作特征曲线下面积，灵敏度为81.5%，特异性为71.4%.Conclusions联合分析的生化档案的两种血液成分，而不是一个单一的生物标志物是一个很有前途的策略，为早期发现结直肠癌。需要进一步的研究来验证我们的初步临床结果。
Background Early detection of colorectal cancer (CRC) can reduce mortality and morbidity. Current screening methods include colonoscopy and stool tests, but a simple low-cost blood test would increase compliance. This preliminary study assessed the utility of analyzing the entire bio-molecular profile of peripheral blood mononuclear cells (PBMCs) and plasma using Fourier transform infrared (FTIR) spectroscopy for early detection of CRC.Methods Blood samples were prospectively collected from 62 candidates for CRC screening/diagnostic colonoscopy or surgery for colonic neoplasia. PBMCs and plasma were separated by Ficoll gradient, dried on zinc selenide slides, and placed under a FTIR microscope. FTIR spectra were analyzed for biomarkers and classified by principal component and discriminant analyses. Findings were compared among diagnostic groups.Results Significant changes in multiple bands that can serve as CRC biomarkers were observed in PBMCs (p = similar to 0.01) and plasma (p = similar to 0.0001) spectra. There were minor but statistically significant differences in both blood components between healthy individuals and patients with benign polyps. Following multivariate analysis, the healthy individuals could be well distinguished from patients with CRC, and the patients with benign polyps were mostly distributed as a distinct subgroup within the overlap region. Leave-one-out cross-validation for evaluating method performance yielded an area under the receiver operating characteristics curve of 0.77, with sensitivity 81.5 % and specificity 71.4 %.Conclusions Joint analysis of the biochemical profile of two blood components rather than a single biomarker is a promising strategy for early detection of CRC. Additional studies are required to validate our preliminary clinical results.