Mechanism of xanthophyll-cycle-mediated photoprotection in Cerasus humilis seedlings under water stress and subsequent recovery
Mechanism of xanthophyll-cycle-mediated photoprotection in Cerasus humilis seedlings under water stress and subsequent recovery
复制标题
水分胁迫下樱桃幼苗叶黄素循环介导的光保护机制及后续恢复
DOI：
10.1007/s11099-011-0065-4
复制
发表时间：
2011-12-01
期刊：
影响因子：
2.7
通讯作者：
Ma, X. L.
中科院分区：
文献类型：
--
作者：
Song, X. S.;Shang, Z. W.;Ma, X. L.
周期性干旱波动是西北地区普遍存在的现象。短句来源本研究分析了适应性较强的矮灌木品种中国矮樱桃（Cerasus humilis）幼苗对水分胁迫（WS）的响应：叶片相对含水量（LRWC）、净光合速率（PN）、PSII光化学最大量子产量（Fv/Fm）和PSII光化学有效量子产量（ΦPSII）随水分亏缺的增加而降低。Chl荧光的非光化学猝灭（NPQ）显著增加，紫黄质(V) +花黄质(A) +玉米黄质(Z)含量显著增加。在整个水平上，WS幼苗光合色素组成变化不显著。而黄叶素循环色素的去环氧化状态[(Z+0.5A)/VAZ]在WS幼苗中普遍较高。二硫苏糖醇（DTT）对Z的去环氧化反应有显著的抑制作用，而氨基葡萄糖（Gla）对Z的去环氧化反应的影响可以忽略不计；紫黄质去环氧酶（VDE）和玉米黄质环氧酶（ZEP）表达谱在WS期间略有稳定上调，表明它们在转录后水平上受到调控。
Periodic drought fluctuation is a common phenomenon in Northwest China. We analyzed the response of Chinese dwarf cherry (Cerasus humilis) seedlings, a dwarf shrub species with considerably strong adaptabilities, exposed to water stress (WS) by withholding water for 21 d, and subsequent recovery of 7 d. Leaf relative water content (LRWC), net photosynthetic rate (PN), maximal quantum yield of PSII photochemistry (Fv/Fm), and effective quantum yield of PSII photochemistry (ΦPSII) decreased with increasing water deficit. In contrast, the nonphotochemical quenching of Chl fluorescence (NPQ) significantly increased, as well as the amounts of violaxanthin (V) + antheraxanthin (A) + zeaxanthin (Z). In the whole levels, the photosynthetic pigment composition did not display significant changes in WS seedlings. However, the de-epoxidation state of xanthophyll cycle pigments [(Z+0.5A)/VAZ ] generally exhibited higher values in WS seedlings. The significant inhibition of de-epoxidation by dithiothreitol (DTT) and negligible changes of epoxidation of Z by glucosamine (Gla) were both observed; the slight but stably upregulated transcript level of violaxanthin de-epoxidase (VDE) and downregulated zeaxanthin epoxidase (ZEP) expression profile were found during WS period, indicating that they were regulated on post-transcript levels.