A pre-docking role for microtubules in insulin-stimulated glucose transporter 4 translocation
A pre-docking role for microtubules in insulin-stimulated glucose transporter 4 translocation
复制标题
微管在胰岛素刺激的葡萄糖转运蛋白 4 易位中的预对接作用
DOI：
10.1111/j.1742-4658.2007.06232.x
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发表时间：
2008-02-01
期刊：
影响因子：
5.4
通讯作者：
Xu, Tao
中科院分区：
文献类型：
--
作者：
Chen, Yu;Wang, Yan;Xu, Tao
胰岛素通过诱导葡萄糖转运蛋白4（GLUT 4）从细胞内转移到质膜来刺激葡萄糖摄取。GLUT 4储存囊泡如何从细胞内部转移到质膜仍有待阐明。在本研究中，GLUT 4储存囊泡的细胞内运输和其在质膜上的对接动力学进行了全面研究，在单囊泡水平的控制和微管破坏3 T3-L1脂肪细胞的延时全内反射荧光显微镜。它表明，微管破坏基本上抑制胰岛素刺激的GLUT 4易位。详细的分析表明，微管破坏阻断了GLUT 4储存囊泡的招募到质膜下，并取消了它们在质膜上的对接。这些数据表明，GLUT 4储存囊泡转运到质膜发生沿着微管，这种转运是胰岛素刺激的GLUT 4易位的必要条件。
Insulin stimulates glucose uptake by inducing translocation of glucose transporter 4 (GLUT4) from intracellular resides to the plasma membrane. How GLUT4 storage vesicles are translocated from the cellular interior to the plasma membrane remains to be elucidated. In the present study, intracellular transport of GLUT4 storage vesicles and the kinetics of their docking at the plasma membrane were comprehensively investigated at single vesicle level in control and microtubule-disrupted 3T3-L1 adipocytes by time-lapse total internal reflection fluorescence microscopy. It is demonstrated that microtubule disruption substantially inhibited insulin-stimulated GLUT4 translocation. Detailed analysis reveals that microtubule disruption blocked the recruitment of GLUT4 storage vesicles to underneath the plasma membrane and abolished the docking of them at the plasma membrane. These data suggest that transport of GLUT4 storage vesicles to the plasma membrane takes place along microtubules and that this transport is obligatory for insulin-stimulated GLUT4 translocation.