SILAC-based proteomic analysis to investigate the impact of amyloid precursor protein expression in neuronal-like B103 cells.
SILAC-based proteomic analysis to investigate the impact of amyloid precursor protein expression in neuronal-like B103 cells.
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DOI：
10.1002/elps.201200251
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发表时间：
2012-12
期刊：
影响因子：
2.9
通讯作者：
Padmanabhan, Jaya
中科院分区：
文献类型：
--
作者：
Chaput, Dale;Kirouac, Lisa Hornbeck;Bell-Temin, Harris;Stevens, Stanley M., Jr.;Padmanabhan, Jaya
阿尔茨海默病（AD）是老年人中最常见的痴呆形式。淀粉样蛋白斑块的形成是由淀粉样前体蛋白（APP）衍生的淀粉样β肽聚集而成，被认为是导致AD病理学的标志性过程之一;然而，APP在斑块形成和AD发病机制中的确切作用尚未确定。使用细胞培养物中氨基酸的稳定同位素标记（SILAC）和质谱法，将APP无效的大鼠神经元样B103细胞的蛋白质表达谱与表达APP同种型APP-695的B103-695细胞进行比较。在3个生物重复中鉴定了总共2，979个独特的蛋白质组，并且在总共100个非冗余蛋白质中鉴定了显著的蛋白质表达变化。与鉴定的差异表达蛋白相关的一些顶级生物学功能包括细胞组装、组织和形态、细胞周期、脂质代谢、蛋白质折叠和翻译后修饰。我们报告了神经元样细胞中APP-695表达影响的几种新的生物学途径，并为研究与APP表达和加工相关的分子机制改变以及对AD病理学的贡献提供了额外的框架。
Alzheimer’s disease (AD) is the most prevalent form of dementia in the elderly. Amyloid plaque formation through aggregation of the amyloid beta peptide derived from amyloid precursor protein (APP) is considered one of the hallmark processes leading to AD pathology; however, the precise role of APP in plaque formation and AD pathogenesis is yet to be determined. Using stable isotope labeling by amino acids in cell culture (SILAC) and mass spectrometry, protein expression profiles of APP null, rat neuronal-like B103 cells were compared to B103-695 cells which express the APP isoform, APP-695. A total of 2,979 unique protein groups were identified among 3 biological replicates and significant protein expression changes were identified in a total of 100 non-redundant proteins. Some of the top biological functions associated with the differentially expressed proteins identified include cellular assembly, organization and morphology, cell cycle, lipid metabolism, protein folding, and posttranslational modifications. We report several novel biological pathways influenced by APP-695 expression in neuronal-like cells and provide additional framework for investigating altered molecular mechanisms associated with APP expression and processing and contribution to AD pathology.
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影响因子：
15.1
作者：
Judge M;Hornbeck L;Potter H;Padmanabhan J
通讯作者：
Padmanabhan J
影响因子：
3.5
作者：
Huttunen, Henri J.;Greco, Christopher;Kovacs, Dora M.
通讯作者：
Kovacs, Dora M.
影响因子：
4.2
作者：
Hernandez-Ortega, Karina;Ferrera, Patricia;Arias, Clorinda
通讯作者：
Arias, Clorinda
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
64.8
作者：
Duff, K;Eckman, C;Younkin, S
通讯作者：
Younkin, S