Increased heat shock protein 70 levels in induced sputum and plasma correlate with severity of asthma patients
Increased heat shock protein 70 levels in induced sputum and plasma correlate with severity of asthma patients
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诱导痰和血浆中热休克蛋白 70 水平升高与哮喘患者的严重程度相关
DOI：
10.1007/s12192-011-0271-9
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发表时间：
2011-11-01
影响因子：
3.8
通讯作者：
Zou Fei
中科院分区：
文献类型：
--
作者：
Hou Changchun;Zhao Haijin;Zou Fei
高迁移率族蛋白1（HMGB 1）和热休克蛋白70（HSP 70）等损伤相关分子模式分子参与了哮喘的发病机制。本研究的目的是检测哮喘患者诱导痰和血浆中HSP 70的浓度，以确定其与气道阻塞的关系。34名健康对照和56名性别和年龄相匹配的持续性支气管哮喘患者入选本研究。在诱导痰液前进行肺量测定。用ELISA试剂盒检测诱导痰和血浆中HSP 70的水平。哮喘患者痰液和血浆中HSP 70浓度显著高于对照组（痰液，（0.88 ng/ml（0.27-1.88 ng/ml）vs 0.42 ng/ml（0.18-0.85 ng/ml），p< 0.001;血浆，（0.46 ng/ml（0.20-0.98 ng/ml）vs 0.14 ng/ml（0.11-0.37 ng/ml），p< 0.001），并与1 s用力呼气容积（FEV 1）、FEV 1（预测百分比）和FEV 1/FVC。嗜酸性粒细胞性哮喘与非嗜酸性粒细胞性哮喘患者的HSP 70水平无显著差异。血浆中HSP 70水平与中性粒细胞计数呈正相关，诱导痰中HSP 70水平与淋巴细胞计数呈正相关。在多因素分析中，痰液HSP 70的独立预测因素是疾病和疾病严重程度，而不是吸烟，年龄或性别，血浆HSP 70的独立预测因素也是疾病和疾病严重程度。结论：诱导痰和血浆HSP 70可作为评估哮喘患者气道阻塞程度的一个有用指标。然而，需要进一步的研究来确定循环和痰中HSP 70在哮喘发病机制中的作用。
Damage-associated molecular pattern molecules such as high-mobility group box 1 protein (HMGB1) and heat shock protein 70 (HSP70) have been implicated in the pathogenesis of asthma. The aim of our study was to examine the induced sputum and plasma concentrations of HSP70 in asthmatic patients to determine their relationship with airway obstruction. Thirty-four healthy controls and 56 patients with persistent bronchial asthma matched for gender and age were enrolled in this study. Spirometry measurements were performed before sputum induction. HSP70 levels in induced sputum and plasma were measured using the ELISA Kit. Sputum and plasma concentrations of HSP70 in asthmatics patients were significantly higher than that in control subjects (sputum, (0.88 ng/ml (0.27–1.88 ng/ml) versus 0.42 ng/ml (0.18–0.85 ng/ml), p < 0.001); plasma, (0.46 ng/ml (0.20–0.98 ng/ml) versus 0.14 ng/ml (0.11–0.37 ng/ml), p < 0.001) and were significantly negatively correlated with forced expiratory volume in 1 s (FEV1), FEV1 (percent predicted), and FEV1/FVC in all 90 participants and 56 patients with asthma. There were no significant differences in HSP70 levels between patients with eosinophilic and non-eosinophilic asthma. HSP70 levels in plasma were positively correlated with neutrophil count, and HSP70 levels in induced sputum were positively correlated with lymphocyte count. In multivariate analysis, independent predictors of sputum HSP70 were diseases and disease severity but not smoking, age, or gender, and independent predictors of plasma HSP70 were also diseases and disease severity. In conclusion, this study indicates that induced sputum and plasma HSP70 could serve as a useful marker for assessing the degree of airway obstruction in patients with asthma. However, further investigation is needed to establish the role of circulating and sputum HSP70 in the pathogenesis of asthma.