Effects of alkylphenols on bone metabolism in vivo and in vitro
Effects of alkylphenols on bone metabolism in vivo and in vitro
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DOI：
10.1016/j.toxlet.2008.06.863
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发表时间：
2008-09-10
影响因子：
3.5
通讯作者：
Ishihara, Yoko
中科院分区：
文献类型：
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作者：
Hagiwara, Hiromi;Sugizaki, Toshinori;Ishihara, Yoko
烷基酚是内分泌干扰物，在各种野生动物中表现出雌激素样作用。然而，关于这些化学物质对骨代谢的作用的信息很少。本研究通过体外实验和体外实验，探讨了壬基酚（NP）和辛基酚（OP）等烷基酚类化合物对破骨细胞和成骨细胞骨形成的影响。NP和OP剂量依赖性地抑制小鼠脾细胞或小鼠骨髓细胞与ST 2细胞共培养物中抗酒石酸酸性磷酸酶阳性多核细胞（破骨细胞）的形成。然而，10（-9）M至10（-6）M的β-雌二醇不影响这一过程。相反，这两种化合物都不影响大鼠颅骨成骨细胞样细胞（ROB细胞）的增殖和分化。在交配后10天、12天和14天分别给孕鼠皮下注射NP或OP（0.1mg/kg体重），在交配后17.5天胎鼠出现胸骨节骨钙化刺激。我们的研究结果表明，烷基酚通过非雌激素效应对骨形成具有关键作用。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
Alkylphenols are endocrine disruptors that show estrogen-like effects in various wildlife species. However, little information is available about the action of these chemicals on bone metabolism. We investigated the effects of alkylphenols, such as nonylphenol (NP) and octylphenol (OP), on the formation ofbone using several culture systems for osteoclasts and osteoblasts, as well as in vivo experiments. NP and OP dose-dependently inhibited the formation of tartrate-resistant acid phosphatase-positive multinucleated cells (osteoclasts) in cocultures of mouse spleen cells or mouse bone marrow cells with ST2 cells. However, beta-estradiol at 10(-9) M to 10(-6) M did not affect this process. In contrast, neither compound affected the proliferation and differentiation of rat calvarial osteoblast-like cells (ROB cells). When NP or OP (0.1 mg/kg body weight) was administered subcutaneously to pregnant mice at 10 days, 12 days and 14 days Post-coitus, fetuses at 17.5 days post-coitus showed stimulation of sternebrae bone calcification. Our findings suggest that alkylphenols have critical effects on the formation of bone by non-estrogenic effects. (C) 2008 Elsevier Ireland Ltd. All rights reserved.