A single, continuous metric to define tiered serum neutralization potency against HIV.
A single, continuous metric to define tiered serum neutralization potency against HIV.
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DOI：
10.7554/elife.31805
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发表时间：
2018-01-19
期刊：
影响因子：
7.7
通讯作者：
Roederer M
中科院分区：
文献类型：
--
作者：
Hraber P;Korber B;Wagh K;Montefiori D;Roederer M
HIV-1包膜（Env）变异体根据其中和敏感性表型分为不同的等级。这有助于认识到第1级中和反应可以很容易地引起，但不能防止新的感染。3级病毒对中和最不敏感。由于大多数流行病毒是2级病毒，因此需要引起针对它们的中和反应的疫苗。虽然病毒的分级被广泛使用，但需要一种方法来以可比的方式对血清或抗体中和反应进行评级。中和结果的逻辑回归以连续的分层量表总结血清或抗体效价。它还检验了中和评分的显著性，以表明血清应答不依赖于病毒等级的病例。该方法可以标准化来自不同病毒组的结果，并可能导致高通量检测，该检测评估单一血清稀释液，而不是稀释系列，以更有效地利用有限的资源来筛选疫苗接种者的样本。
HIV-1 Envelope (Env) variants are grouped into tiers by their neutralization-sensitivity phenotype. This helped to recognize that tier 1 neutralization responses can be elicited readily, but do not protect against new infections. Tier 3 viruses are the least sensitive to neutralization. Because most circulating viruses are tier 2, vaccines that elicit neutralization responses against them are needed. While tier classification is widely used for viruses, a way to rate serum or antibody neutralization responses in comparable terms is needed. Logistic regression of neutralization outcomes summarizes serum or antibody potency on a continuous, tier-like scale. It also tests significance of the neutralization score, to indicate cases where serum response does not depend on virus tiers. The method can standardize results from different virus panels, and could lead to high-throughput assays, which evaluate a single serum dilution, rather than a dilution series, for more efficient use of limited resources to screen samples from vaccinees.