Automated ion-exchange chromatography of uronic acids and uronic acid containing oligosaccharides.
Automated ion-exchange chromatography of uronic acids and uronic acid containing oligosaccharides.
复制标题
糖醛酸和含糖醛酸寡糖的自动离子交换色谱。
DOI：
10.1016/0003-2697(68)90362-x
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发表时间：
1968
影响因子：
2.9
通讯作者：
M. L. Spach
中科院分区：
文献类型：
--
作者：
L. Fransson;L. Rodén;M. L. Spach
介绍了一种用于糖醛酸和含糖醛酸寡糖分离的自动化阴离子交换色谱法。该方法已被应用，特别是，L-艾杜糖醛酸和D-葡萄糖醛酸的硫酸皮肤素的水解产物中的分离和定量，并已确定的糖醛酸的最佳收率所需的水解条件。而糖醛酸和醛二糖糖醛酸成功地分离与甲酸洗脱，透明质酸寡糖，特别是，高度带电的硫酸软骨素寡糖，更方便地解决在氯化物体系中的非线性氯化锂梯度洗脱。该系统还用于分离由软骨素酶消化软骨素和硫酸软骨素获得的不饱和和饱和二糖。通过Technicon糖色谱系统的自动邻苯二酚硫酸法分析柱流出物。
An automated anion-exchange chromatographic procedure for the separation of uronic acids and uronic acid containing oligosaccharides is described. The method has been applied, in particular, to the separation and quantitation of l-iduronic and d-glucuronic acid in hydrolyzates of dermatan sulfate, and the hydrolysis conditions required for optimum yield of uronic acids have been determined. Whereas uronic acids and aldobiuronic acids were successfully separated by elution with formic acid, hyaluronic acid oligosaccharides and, especially, the highly charged chondroitin sulfate oligosaccharides were more conveniently resolved in a chloride system by elution with a nonlinear LiCl gradient. This system was also employed for the separation of unsaturated and saturated disaccharides obtained by chondroitinase digestion of chondroitin and chondroitin sulfate. The effluent from the columns was analyzed by the automated orcinolsulfuric acid method of the Technicon sugar chromatography system.