Assignment of 112 microsatellite markers to 23 chromosome 11 subregions delineated by somatic hybrids: comparison with the genetic map.
Assignment of 112 microsatellite markers to 23 chromosome 11 subregions delineated by somatic hybrids: comparison with the genetic map.
复制标题
将 112 个微卫星标记分配给体细胞杂交所描绘的 23 个 11 号染色体亚区：与遗传图谱的比较。
作者：
P. Couillin;E. le Guern;A. Vignal;C. Fizames;N. Ravisé;D. Delportes;I. Reguigne;M. Rosier;C. Junien;V. van Heyningen
使用一组25个体细胞杂种，我们区域定位了112个微卫星标记产生的Généthon和分配到染色体11。其中74人的遗传图谱是使用连锁分析的8个最大的CEPH（中心德研究杜多态性人类）的家庭。它们在11号染色体上排列整齐，平均距离为2.1cM。这些微卫星的遗传顺序和物理分配之间的紧密相关性加强了遗传图谱数据。通过PCR方法使用标准化方案鉴定的这些新定位的标记代表了用于绘制YAC克隆和建立YAC重叠群以及用于研究与特定基因和/或11号染色体的胚/体细胞重排相关的遗传疾病或癌症的有用工具。
Using a panel of 25 somatic cell hybrids, we have regionally localized 112 microsatellite markers generated by Généthon and assigned to chromosome 11. A genetic map of 74 of them was produced using linkage analysis of the eight largest CEPH (Centre d'Etude du Polymorphisme Humain) families. They could be ordered on chromosome 11 with an average distance of 2.1 cM. The tight correlation observed between the genetic order and the physical assignment of these microsatellites reinforces the genetic map data. These newly localized markers identified by the PCR method using a standardized protocol represent useful tools for mapping YAC clones and establishing YAC contigs and for studying genetic diseases or cancers associated with specific genes and/or germinal/somatic rearrangements of chromosome 11.