Molecular characterization of the song control nucleus HVC in Bengalese finch brain
Molecular characterization of the song control nucleus HVC in Bengalese finch brain
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DOI：
10.1016/j.brainres.2010.09.014
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发表时间：
2010-11-11
期刊：
影响因子：
2.9
通讯作者：
Okanoya, Kazuo
中科院分区：
文献类型：
--
作者：
Kato, Masaki;Okanoya, Kazuo
鸣禽有一种特殊的神经底物，用于习得发声，称为歌声回路，由大脑中的几个歌声核团组成。歌曲控制核心HVC(基于字母的名称)是歌曲学习和发声运动通路的交叉点。了解在HVC中表达的基因类型对于理解HVC的分子方面是必不可少的。在无声条件下，HVC的基因表达显示了歌唱所必需的能力。为了研究这一点，我们使用孟加拉雀(Lonchura Striata Var)的内部cDNA微阵列，比较了HVC及其邻近组织，以寻找宋氏核HVC的分子特异性。家庭式)。我们的微阵列分析显示，与邻近组织相比，70个基因在HVC中差异表达。我们通过原位杂交研究了27个在HVC中被富含或抑制的基因。我们发现多种钙结合蛋白(如CAPS2、小白蛋白和ATH)在HVC中富含。同时，与幼年HVC相比，成年HVC的可塑性相关基因(如CAMK2A和MAP2K1)的表达水平较低。HVC在歌曲学习中起着重要作用，但我们的结果表明，该核团的可塑性在成年期可能受到抑制。我们的发现为HVC的分子特征提供了新的信息。(C)2010爱思唯尔B.V.保留所有权利。
Songbirds have a specialized neural substrate for learned vocalization, called the song circuit, which consists of several song nuclei in the brain. The song control nucleus HVC (a letter-based name) is the intersection point of the song learning and vocal motor pathways. Knowledge of the types of genes expressed in the HVC is essential in understanding the molecular aspects of the HVC. Gene expression in the HVC under silent conditions shows the competence necessary for singing. To investigate this, we compared the HVC with its adjacent tissues in searching for the molecular specificities of the song nucleus HVC using an in-house cDNA microarray of the Bengalese finch (Lonchura striata var. domestica). Our microarray analysis revealed that 70 genes were differentially expressed in the HVC compared with the adjacent tissue. We investigated 27 of the microarray-selected genes that were enriched or repressed in the HVC by in situ hybridization. We found that multiple calcium-binding proteins (e.g., CAPS2, parvalbumin and ATH) were enriched in the HVC. Meanwhile, the adult HVC showed low expression levels of plasticity-related genes (e.g., CAMK2A and MAP2K1) compared with the juvenile HVC. The HVC plays an important role during song learning, but our results suggest that the plasticity of this nucleus may be suppressed during adulthood. Our findings provide new information about the molecular features that characterize the HVC. (C) 2010 Elsevier B.V. All rights reserved.