Prevalence of bacteria of division TM7 in human subgingival plaque and their association with disease
Prevalence of bacteria of division TM7 in human subgingival plaque and their association with disease
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DOI：
10.1128/aem.69.3.1687-1694.2003
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发表时间：
2003-03-01
影响因子：
4.4
通讯作者：
Relman, DA
中科院分区：
文献类型：
--
作者：
Brinig, MM;Lepp, PW;Relman, DA
在人类口腔中检测到未经培养的细菌分裂TM7成员，但关于其在该地点的流行程度和多样性的信息很少。从健康部位和表现牙周病不同阶段的部位采集的人类龈下菌斑样本进行了TM7细菌的分析。96%的样品中检测到TM7核糖体DNA (rDNA)，通过实时定量PCR检测，平均约占样品中所有细菌rDNA的0.3%。鉴定了该分支的两个新的种型，并通过荧光原位杂交发现该分支的成员表现出丝状形态。轻度牙周炎部位TM7 rDNA相对于细菌总rDNA的丰富度（0.54% +/- 0.1%）高于健康部位（0.21% 0.05%,P < 0.01）和重度牙周炎部位（0.29% +/- 0.06%,P < 0.05）。在18个健康样本中的1个和58个疾病样本中的38个中检测到一个分裂亚群，即I025种型。这些数据表明，这种类型，以及一般的TM7细菌分裂，可能在导致牙周炎的多因素过程中发挥作用。
Members of the uncultivated bacterial division TM7 have been detected in the human mouth, but little information is available regarding their prevalence and diversity at this site. Human subgingival plaque samples from healthy sites and sites exhibiting various stages of periodontal disease were analyzed for the presence of TM7 bacteria. TM7 ribosomal DNA (rDNA) was found in 96% of the samples, and it accounted for approximately 0.3%, on average, of all bacterial rDNA in the samples as determined by real-time quantitative PCR. Two new phylotypes of this division were identified, and members of the division were found to exhibit filamentous morphology by fluorescence in situ hybridization. The abundance of TM7 rDNA relative to total bacterial rDNA was higher in sites with mild periodontitis (0.54% +/- 0.1%) than in either healthy sites (0.21% 0.05%, P < 0.01) or sites with severe periodontitis (0.29% +/- 0.06%, P < 0.05). One division subgroup, the I025 phylotype, was detected in 1 of 18 healthy samples and 38 of 58 disease samples. These data suggest that this phylotype, and the TM7 bacterial division in general, may play a role in the multifactorial process leading to periodontitis.