Evidence for the autoregulation of hormone secretion by prolactin.
Evidence for the autoregulation of hormone secretion by prolactin.
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催乳素自动调节激素分泌的证据。
DOI：
10.1210/endo-104-1-97
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发表时间：
1979
期刊：
影响因子：
4.8
通讯作者：
E. Rennels
中科院分区：
文献类型：
--
作者：
D. Herbert;H. Ishikawa;E. Rennels
将来自垂体细胞的2B 8克隆株的细胞（先前已报道其仅产生PRL）与不同量的绵羊PRL（oPRL）（范围为0.01- 1000 ng/ml）一起孵育。1小时后，在来自用所有剂量的oPRL孵育的细胞的培养基中测量到显著更少的PRL。该抑制与剂量相关。用Hank平衡盐溶液冲洗细胞5次，随后将其置于无PRL培养基中再培养1小时。先前暴露于1- 1000 ng/ml oPRL的那些细胞发生激素分泌过多。预先暴露于0.01- 0.1ng/ml的细胞培养液中的PRL与对照培养液中的PRL相似。在一些实验中，在两个1小时孵育期结束时测定PRL的细胞浓度。在初始暴露于oPRL后，仅在与1- 1000 ng/ml oPRL孵育的那些组中观察到细胞内激素的增加。相反，在冲洗细胞并置于不含PRL的培养基中后，除先前暴露于100 ng/ml oPRL的组外，所有组中PRL的细胞浓度均未发生变化。这些数据表明，PRL可以通过直接作用于垂体前叶的催乳素来抑制其自身的分泌。
The cells from the 2B8 clonal strain of pituitary cells which previously have been reported to produce only PRL were incubated with varying amounts of ovine PRL (oPRL) ranging from 0.01--1000 ng/ml. After 1 h, significantly less PRL was measured in culture media from cells incubated with all doses of oPRL employed. This inhibition was dose related. The cells were rinsed five times with Hank's balanced salt solution and subsequently placed into PRL-free media for an additional hour. Hypersecretion of hormone occurred from those cells previously exposed to 1--1000 ng/ml oPRL. The PRL in the media from cells previously exposed to 0.01--0.1 ng/ml was similar to that in the control culture. In some experiments, the cellular concentration of PRL was determined at the end of each of the two 1-h incubation periods. After the initial exposure to oPRL, an increase in intracellular hormone was observed only in those groups incubated with 1--1000 ng/ml oPRL. In contrast, after the cells were rinsed and placed into PRL-free medium, the cellular concentration of PRL was unchanged in all groups except the group previously exposed to 100 ng/ml oPRL. These data indicate that PRL can inhibit its own secretion through direct action on the lactotrophs of the anterior pituitary gland.