Organ-specific IgM autoantibodies to liver, heart and brain in man: generalized occurrence and possible functional significance in normal individuals, and studies in patients with multiple sclerosis.
Organ-specific IgM autoantibodies to liver, heart and brain in man: generalized occurrence and possible functional significance in normal individuals, and studies in patients with multiple sclerosis.
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人类肝脏、心脏和大脑的器官特异性 IgM 自身抗体：正常个体中的普遍发生和可能的功能意义，以及多发性硬化症患者的研究。
DOI：
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发表时间：
1981
影响因子：
4.6
通讯作者：
J. Fabre
中科院分区：
文献类型：
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作者：
A. Daar;J. Fabre
在本文中，我们使用一种敏感的 125I 抗免疫球蛋白结合测定法（最近适用于以组织匀浆为靶标）来证明正常人血清中针对脑、肝和心脏的自身抗体。定量吸收分析表明，检测到的自身抗原在每种情况下都是器官特异性的，并且脑自身抗原在大脑皮层、大脑白质和小脑皮层上以等量存在。通过凝胶过滤研究和实验表明，自身抗体本质上是 IgM，其中 IgM 被还原为单体，并且发现在 4、20 和 37 摄氏度下结合效果相同。与大鼠和狗的大脑、肝脏和心脏的交叉反应是不可预测的，通常很弱。肾匀浆的平行研究未能检测到抗肾脏自身抗体，但冰冻切片的免疫荧光研究显示，正常肾脏中存在大量免疫球蛋白，主要位于肾小球和肾小管基底膜上，这增加了存在肾脏自身抗体但自身抗原位点位于体内的可能性。多发性硬化症患者的血清与脑匀浆的结合与正常血清没有区别，五名多发性硬化症患者的脑脊液根本不与脑匀浆结合。讨论了正常人中多种IgM自身抗体的理论和实际意义以及自身抗体的器官特异性。
In this paper we use a sensitive, 125I-anti-immunoglobulin-binding assay, recently adapted for use with tissue homogenates as targets, to demonstrate autoantibodies to brain, liver and heart in the sera of normal persons. Quantitative absorption analyses demonstrated that the autoantigens detected were in each case organ-specific, and the brain autoantigen was shown to be present in equal amounts on cerebral cortex, cerebral white matter and cerebellar cortex. The autoantibodies were shown to be IgM in nature by gel filtration studies and experiments where IgM was reduced to monomers, and were found to bind equally well at 4, 20 and 37 degrees C. Cross-reactions with brain, liver and heart of rat and dog were unpredictable and usually weak. Parallel studies with kidney homogenates failed to detect anti-kidney autoantibodies, but immunofluorescence studies on frozen sections revealed large amounts of immunoglobulin in normal kidneys, mainly on glomerular and tubular basement membranes, raising the possibility that autoantibodies to kidney are present but that the autoantigen sites are situated in vivo. Sera from patients with multiple sclerosis were indistinguishable from normal sera in their binding to brain homogenate, and CSF from five patients with multiple sclerosis did not bind at all to brain homogenate. The theoretical and practical significance of multiple IgM autoantibodies in normal persons, and the organ specificity of the autoantibodies, is discussed.