Regions of variant histone His2AvD required for Drosophila development
Regions of variant histone His2AvD required for Drosophila development
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DOI：
10.1038/21436
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发表时间：
1999-06-17
期刊：
影响因子：
64.8
通讯作者：
Tremethick, DJ
中科院分区：
文献类型：
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作者：
Clarkson, MJ;Wells, JRE;Tremethick, DJ
建立一个独特的染色体结构域以实现特定功能的一种方法是将特定的组蛋白变体局部掺入核小体中。H2 AZ是组蛋白H2 A的一种变体，是黑腹果蝇（1）、嗜热四膜虫（2）和小鼠（R. Faast等人，准备中）。为了寻找果蝇H2 AZ（His 2AvD，也称为H2 AvD）的独特功能，其基本功能所需的，我们进行了氨基酸交换实验，其中果蝇His 2AvD独特的残基被替换为等同位置的果蝇H2A.1残基。将编码该组蛋白的修饰版本的突变的His 2AvD基因转化到果蝇中，并测试其拯救无效突变致死性的能力。我们发现，His 2AvD的独特功能不驻留在其组蛋白折叠，但在其羧基末端结构域。这个C-末端区域映射到H2 A中的一个短α-螺旋，该螺旋深埋在核小体核心内。
One way in which a distinct chromosomal domain could be established to carry out a specialized function is by the localized incorporation of specific histone variants into nucleosomes. H2AZ, one such variant of the histone protein H2A, is required for the survival of Drosophila melanogaster(1), Tetrahymena thermophila(2) and mice (R. Faast et al., in preparation). To search for the unique features of Drosophila H2AZ (His2AvD, also referred to as H2AvD) that are required for its essential function, we have performed amino-acid swap experiments in which residues unique to Drosophila His2AvD were replaced with equivalently positioned Drosophila H2A.1 residues. Mutated His2AvD genes encoding modified versions of this histone were transformed into Drosophila and tested for their ability to rescue null-mutant lethality. We show that the unique feature of His2AvD does not reside in its histone fold but in its carboxy-terminal domain. This C-terminal region maps to a short alpha-helix in H2A that is buried deep inside the nuceleosome core.