Controlling Collective Motion of Kinesin-Driven Microtubules via Patterning of Topographic Landscapes
Controlling Collective Motion of Kinesin-Driven Microtubules via Patterning of Topographic Landscapes
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DOI：
10.1021/acs.nanolett.1c03952
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发表时间：
2021-12-07
期刊：
影响因子：
10.8
通讯作者：
Maeda, Yusuke T.
中科院分区：
文献类型：
--
作者：
Araki, Shunya;Beppu, Kazusa;Maeda, Yusuke T.
生物分子马达蛋白通过消耗ATP水解获得的化学能产生力，在组织活细胞中的细胞骨架结构中起关键作用。控制细胞骨架结构的能力将有利于可编程蛋白质图案化;然而，我们目前的知识是有限的，因为用于控制图案形成的工程方法不发达。在这里，我们展示了驱动蛋白马达驱动的微管（MT）的自组装模式的控制，通过设计制造的微孔中的边界形状。通过控制边界形状定义的滑动微球的碰撞角度，可以控制微球的自组装形成突出的束和桥图案。通过自推进杆理论的证实，我们进一步表明，MT的排列决定了组装模式之间的过渡，提供了一个蓝图，以重建微通道中的桥梁结构。我们的研究结果介绍了定制的自组织的细胞骨架和马达蛋白的纳米技术应用。
Biomolecular motor proteins that generate forces by consuming chemical energy obtained from ATP hydrolysis play pivotal roles in organizing cytoskeletal structures in living cells. An ability to control cytoskeletal structures would benefit programmable protein patterning; however, our current knowledge is limited because of the underdevelopment of engineering approaches for controlling pattern formation. Here, we demonstrate the controlling of self-assembled patterns of microtubules (MTs) driven by kinesin motors by designing the boundary shape in fabricated microwells. By manipulating the collision angle of gliding MTs defined by the boundary shape, the self-assembly of MTs can be controlled to form protruding bundle and bridge patterns. Corroborated by the theory of self-propelled rods, we further show that the alignment of MTs determines the transition between the assembled patterns, providing a blueprint to reconstruct bridge structures in microchannels. Our findings introduce the tailoring of the self-organization of cytoskeletons and motor proteins for nanotechnological applications.