Green tea polyphenol "epigallocatechin-3-gallate", differentially induces apoptosis in CLL B-and T-Cells but not in healthy B-and T-Cells in a dose dependant manner
Green tea polyphenol "epigallocatechin-3-gallate", differentially induces apoptosis in CLL B-and T-Cells but not in healthy B-and T-Cells in a dose dependant manner
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DOI：
10.1016/j.leukres.2016.10.011
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发表时间：
2016-12-01
影响因子：
2.7
通讯作者：
Ghassemifar, Reza
中科院分区：
文献类型：
--
作者：
Cornwall, Scott;Cull, Gavin;Ghassemifar, Reza
B细胞慢性淋巴细胞白血病（CLL）的特征在于CD 5阳性单克隆B细胞的积累，这在很大程度上是由于细胞凋亡的失败。体外研究CLL B细胞的能力一直是一个挑战，并且受到细胞培养系统中CLL B细胞的低活力的阻碍。在这项研究中，我们提出了一种多细胞细胞培养系统，以保持CLL B-细胞在培养60小时的基质细胞饲养层的存在下，结合全白色血细胞制剂的活力。使用这种优化的系统，我们测试并表明，表没食子儿茶素-3-没食子酸酯（EGCG）的浓度范围为25至100 μ g/ml诱导CLL B细胞凋亡，而不影响健康的对照B细胞。此外，结果显示，与健康对照相比，来自CLL患者的T细胞在存在EGCG的情况下经历凋亡。本研究表明，细胞饲养层与全白色血细胞制剂的组合在体外长时间维持B细胞活力。此外，该研究表明，EGCG以剂量依赖性方式差异性地诱导CLL B细胞和T细胞的凋亡，但不诱导健康B细胞和T细胞的凋亡。(C)2016爱思唯尔有限公司版权所有。
B-cell chronic lymphocytic leukaemia (CLL) is characterized by an accumulation of CD5-positive monoclonal B-cells due in large part to a failure of apoptosis. The ability to study CLL B-cells in vitro has always been a challenge and hampered by the low viability of the CLL B-cells in cell culture systems. In this study, we present a multicellular cell culture system to maintain CLL B-cells viable in culture for 60 h in the presence of a stromal cell feeder layer in combination with a whole white blood cell preparation. Using this optimized system, we tested and showed that the addition of epigallocatechin-3-gallate (EGCG) at concentrations ranging from 25 to 100 mu g/ml induced apoptosis in CLL B-cells whilst not affecting healthy control B-cells. Moreover, the results showed that in contrast to healthy controls, T-cells from CLL patients underwent apoptosis in the presence of EGCG. This study demonstrated that the combination of a cell feeder layer with a whole white blood cell preparation maintained B-cell viability in vitro over an extended period of time. In addition, the study showed that EGCG differentially induces apoptosis in CLL B-and T-Cells but not in healthy B-and T-Cells in a dose dependent manner. (C) 2016 Elsevier Ltd. All rights reserved.