A combination of genistein and magnesium enhances the vasodilatory effect via an eNOS pathway and BK(Ca) current amplification.
A combination of genistein and magnesium enhances the vasodilatory effect via an eNOS pathway and BK(Ca) current amplification.
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DOI：
10.1139/cjpp-2014-0306
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发表时间：
2015-04
影响因子：
2.1
通讯作者：
Lina Sun;Yunlong Hou;T. Zhao;Shanshan Zhou;Xiaoran Wang;Liming Zhang;Guichun Yu
中科院分区：
文献类型：
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作者：
Lina Sun;Yunlong Hou;T. Zhao;Shanshan Zhou;Xiaoran Wang;Liming Zhang;Guichun Yu
植物雌激素染料木黄酮（GST）和镁已被独立地证明可以调节血管张力;然而，它们各自的血管舒张作用有限。本研究的目的是检查GST加镁对肠系膜动脉血管张力的联合作用。用等长收缩力法观察GST（0-200 μmol/L）、MgCl 2（0 ~ 4.8mmol/L）及GST + MgCl 2预处理对10 μmol/L苯肾上腺素（PE）预收缩大鼠肠系膜动脉的影响。采用膜片钳法检测BK（Ca）电流。GST引起浓度依赖性和部分内皮依赖性舒张。镁导致血管张力的双重调整。无镁溶液消除了GST在内皮完整和剥脱环中的血管舒张作用。GST（50 μmol/L）+镁（4.8mmol/L）对内皮完整和剥脱的血管环均有较强的舒张作用。一氧化氮合酶（NOS）抑制剂L-N-硝基精氨酸甲酯（L-NAME，100 μmol/L）预处理可明显抑制GST、高镁及GST与镁联合作用的效应。当GST（50 μmol/L）与4.8mmol/LMg（2+）和1.2mmol/LMg（2+）联合作用时，BK（Ca）电流被放大。我们的数据表明，GST加镁提供了增强的血管舒张作用，在大鼠肠系膜动脉相比，观察到的，无论是单独使用，这是相关的eNOS途径和BK（Ca）电流放大。
The phytoestrogen genistein (GST) and magnesium have been independently shown to regulate vascular tone; however, their individual vasodilatory effects are limited. The aim of this study was to examine the combined effects of GST plus magnesium on vascular tone in mesenteric arteries. The effects of pretreatment with GST (0-200 μmol/L), MgCl2 (0-4.8 mmol/L) and GST plus MgCl2 on 10 μmol/L phenylephrine (PE) precontracted mesenteric arteries in rats were assessed by measuring isometric force. BK(Ca) currents were detected by the patch clamp method. GST caused concentration- and partial endothelium-dependent relaxation. Magnesium resulted in dual adjustment of vascular tone. Magnesium-free solution eliminated the vasodilatation of GST in both endothelium-intact and denuded rings. GST (50 μmol/L) plus magnesium (4.8 mmol/L) caused stronger relaxation in both endothelium-intact and denuded rings. Pretreatment with the nitric oxide synthase (NOS) inhibitor L-N-nitroarginine methyl ester (L-NAME, 100 μmol/L) significantly inhibited the effects of GST, high magnesium, and the combination of GST and magnesium. BK(Ca) currents were amplified to a greater extent when GST (50 μmol/L) was combined with 4.8 versus 1.2 mmol/L Mg(2+). Our data suggest that GST plus magnesium provides enhanced vasodilatory effects in rat mesenteric arteries compared with that observed when either is used separately, which was related to an eNOS pathway and BK(Ca) current amplification.