Polarized Wnt Signaling Regulates Ectodermal Cell Fate in Xenopus
Polarized Wnt Signaling Regulates Ectodermal Cell Fate in Xenopus
复制标题
DOI：
10.1016/j.devcel.2014.03.015
复制
发表时间：
2014-04-28
影响因子：
11.8
通讯作者：
Niehrs, Christof
中科院分区：
文献类型：
--
作者：
Huang, Ya-Lin;Niehrs, Christof
细胞如何将极性线索转换为细胞命运说明尚不完全清楚。在这里，我们发现在非洲爪哇早期胚胎中，Wnt/β-catenin和Wnt/PCP信号在这一过程中相互作用。我们发现Wnt辅助受体LRP6不对称地定位于外胚层卵裂球的基侧膜上。LRP6的不对称性是由Wnt/PCP信号控制的，这表明该途径不仅调节平面细胞，而且还调节顶基细胞的极性。在细胞不对称分裂后，LRP6优先分选到深层的外胚层细胞层，并在上皮细胞层变得枯竭。这伴随着深层细胞中Wnt/β-catenin信号的升高，这反过来又促进了它们向纤毛细胞的分化。我们得出结论，协调的Wnt/PCP和Wnt/β-catenin信号转换顶端基极性信息来指定外胚层细胞的命运。
How cells convert polarity cues into cell fate specification is incompletely understood. Here, we show that Wnt/beta-catenin and Wnt/PCP signaling cooperate in this process in early Xenopus embryos. We find that the Wnt coreceptor Lrp6 is asymmetrically localized to the basolateral membrane in ectodermal blastomeres. Lrp6 asymmetry is controlled by Wnt/PCP signaling, indicating that this pathway regulates not only planar- but also apicobasal cell polarity. Following asymmetric cell division, Lrp6 preferentially sorts to the deep ectodermal cell layer and becomes depleted in the epithelial cell layer. This is accompanied by elevated Wnt/beta-catenin signaling in deep cells, which in turn promotes their differentiation into ciliated cells. We conclude that coordinated Wnt/PCP and Wnt/beta-catenin signaling convert apicobasal polarity information to specify ectodermal cell fate.