Filamentous Bacteriophage Promote Biofilm Assembly and Function.
Filamentous Bacteriophage Promote Biofilm Assembly and Function.
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DOI：
10.1016/j.chom.2015.10.013
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发表时间：
2015-11-11
影响因子：
30.3
通讯作者：
Bollyky PL
中科院分区：
文献类型：
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作者：
Secor PR;Sweere JM;Michaels LA;Malkovskiy AV;Lazzareschi D;Katznelson E;Rajadas J;Birnbaum ME;Arrigoni A;Braun KR;Evanko SP;Stevens DA;Kaminsky W;Singh PK;Parks WC;Bollyky PL
生物膜——包裹在富含聚合物的基质中的细菌群落——赋予细菌在病理宿主环境中持续存在的能力，例如囊性纤维化（CF）气道。细菌如何将聚合物组装成生物膜尚不清楚。我们发现，铜绿假单胞菌产生的细胞外基质通过聚合物和丝状 Pf 噬菌体（带负电荷的长丝）之间的熵相互作用自组装成液晶。这种液晶结构通过增加对干燥和抗生素的粘附性和耐受性来增强生物膜功能。 Pf 噬菌体在铜绿假单胞菌临床分离株中普遍存在，并在 CF 痰液中检测到。将 Pf 噬菌体添加到痰聚合物或血清中足以驱动它们快速组装成粘性液晶。 Fd 是大肠杆菌的一种相关噬菌体，具有类似的生物膜构建能力。针对丝状噬菌体或生物膜基质的液晶组织可能代表抗菌策略。
Biofilms - communities of bacteria encased in a polymer-rich matrix- confer bacteria with the ability to persist in pathologic host contexts, such as the cystic fibrosis (CF) airways. How bacteria assemble polymers into biofilms is largely unknown. We find that the extracellular matrix produced by Pseudomonas aeruginosa self-assembles into a liquid crystal through entropic interactions between polymers and filamentous Pf bacteriophages, which are long, negatively charged filaments. This liquid crystalline structure enhances biofilm function by increasing adhesion and tolerance to desiccation and antibiotics. Pf bacteriophages are prevalent amongst P. aeruginosa clinical isolates and were detected in CF sputum. The addition of Pf bacteriophage to sputum polymers or serum was sufficient to drive their rapid assembly into viscous liquid crystals. Fd, a related bacteriophage of Escherichia coli, has similar biofilm-building capabilities. Targeting filamentous bacteriophage or the liquid crystalline organization of the biofilm matrix may represent antibacterial strategies.