FORMATION OF LARGE SCAFFOLD-FREE 3-D AGGREGATES IN A CELL CULTURE DISH BY ULTRASOUND STANDING WAVE TRAPPING
FORMATION OF LARGE SCAFFOLD-FREE 3-D AGGREGATES IN A CELL CULTURE DISH BY ULTRASOUND STANDING WAVE TRAPPING
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DOI：
10.1016/j.ultrasmedbio.2019.01.013
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发表时间：
2019-05-01
影响因子：
2.9
通讯作者：
Takemura, Kenjiro
中科院分区：
文献类型：
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作者：
Nakao, Misa;Imashiro, Chikahiro;Takemura, Kenjiro
模拟体外细胞间相互作用的细胞聚集体对于生物学研究至关重要。本研究介绍了一种使用千赫兹级超声驻波捕获 (USWT) 在临床上普遍存在的细胞培养皿中形成大型无支架 3D 聚集体的方法。我们制造了一个聚集体形成系统，其中通过水将 60 毫米的盘设置在 Langevin 传感器上方。换能器以110.8 kHz激发，然后将C2C12成肌细胞注入培养皿中并捕获在驻波的节点位置。形成的骨料直径和厚度分别为8和2.7毫米，大于之前USWT形成的骨料。此外，我们证实>94%的构成聚集体的细胞存活9小时，并且细胞的蛋白质表达没有显着改变。该方法可用于形成具有高功能性的聚集体，这有助于生物学研究方法的发展。 (C) 2019 作者。由爱思唯尔公司代表世界医学与生物学超声联合会出版。
Cellular aggregates that mimic cell-cell interactions in vitro are essential for biological research. This study introduces a method to form large scaffold-free 3-D aggregates in a clinically ubiquitous cell culture dish using kilohertz-order ultrasound standing wave trapping (USWT). We fabricated an aggregate formation system in which a 60-mm dish was set above a Langevin transducer via water. The transducer was excited at 110.8 kHz, and then C2C12 myoblasts were injected into the dish and trapped at the node position of the standing wave. The diameter and thickness of the formed aggregate were 8 and 2.7 mm, respectively, which are larger than those of aggregates formed previously by USWT. Moreover, we confirmed that >94% of cells constituting the aggregates survived 9 h, and the protein expression of cells was not altered significantly. This method can be applied to form aggregates with high functionality, which contributes to the development of biological research methodology. (C) 2019 The Author(s). Published by Elsevier Inc. on behalf of World Federation for Ultrasound in Medicine & Biology.