Reporting neural activity with genetically encoded calcium indicators
Reporting neural activity with genetically encoded calcium indicators
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DOI：
10.1007/s11068-008-9029-4
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发表时间：
2008-08-01
期刊：
影响因子：
--
通讯作者：
Looger, Loren L.
中科院分区：
文献类型：
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作者：
Hires, S. Andrew;Tian, Lin;Looger, Loren L.
基于重组荧光蛋白的遗传编码钙指示剂（GECIs）已被设计用于观察活细胞和生物体中的钙瞬变。通过观察钙，这些指标也报告神经活动。本文综述了GECI的构建和应用进展，特别是对稀疏动作电位（AP）的体内监测。我们总结了影响GECI绩效的外在和内在因素。一个简单的模型GECI对AP放电的反应证明了这些因素的相对重要性。我们建议一个标准化的协议，以评估GECIs在生理相关的背景下。提出了一种同时进行神经元回路的光学控制和记录的潜在方法。
Genetically encoded calcium indicators (GECIs), based on recombinant fluorescent proteins, have been engineered to observe calcium transients in living cells and organisms. Through observation of calcium, these indicators also report neural activity. We review progress in GECI construction and application, particularly toward in vivo monitoring of sparse action potentials (APs). We summarize the extrinsic and intrinsic factors that influence GECI performance. A simple model of GECI response to AP firing demonstrates the relative significance of these factors. We recommend a standardized protocol for evaluating GECIs in a physiologically relevant context. A potential method of simultaneous optical control and recording of neuronal circuits is presented.