Dose-dependent effects of acute exercise on PKC levels in rat heart: is PKC the heart's prophylactic?
Dose-dependent effects of acute exercise on PKC levels in rat heart: is PKC the heart's prophylactic?
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DOI：
10.1046/j.1365-201x.2003.01131.x
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发表时间：
2003-06-01
期刊：
影响因子：
--
通讯作者：
Korzick, DH
中科院分区：
文献类型：
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作者：
Carson, LD;Korzick, DH
Epidemiological studies have demonstrated that chronic exercise is cardioprotective, and recent evidence from our laboratory suggests a key role for protein kinase C (PKC)-dependent pathways, at least in part, as a cellular basis for this response. However, the dose-response relationship linking exercise volume and the time course of isoform-specific PKC activation are poorly understood.Aim: The purpose of this investigation was to determine the effects of acute exercise of varying durations on PKC subcellular distribution and phosphorylation in the rat left ventricle.Methods: Adult (5 months) male Fischer-344 more rats were subjected to a single bout (OB) or 7 days (SB) of treadmill running (n=6/group; 23 m min(-1), 20 min), and compared with sedentary controls (SED; n=8). Hearts were isolated immediately after [early window (EW); n=3/group] or 24 h after the last exercise bout [late window (LW); n=3/group] in OB and SD, respectively. Total PKC and subcellular distribution for the alpha, delta, epsilon, beta I, and beta II isoforms, as well as phosphorylated (phospho-) PKCepsilon (pSer(729)), PKCalpha (pSer(657)) and PKCdelta (pThr(507)) levels were assessed by western blotting. Protein kinase Cepsilon and PKCalpha mRNA levels were assessed by real time polymerase chain reaction.Results: Following OB, PKCbeta I protein levels were reduced, while total phospho-PKCepsilon (pSer(729)), PKCalpha (pSer(657)) and PKCdelta (pThr(507)) levels were increased during EW (P
