Rho GTPases and cancer cell transendothelial migration.
Rho GTPases and cancer cell transendothelial migration.
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DOI：
10.1007/978-1-61779-442-1_9
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发表时间：
2012
影响因子：
--
通讯作者：
Nicolas Reymond;P. Riou;A. Ridley
中科院分区：
文献类型：
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作者：
Nicolas Reymond;P. Riou;A. Ridley
小Rho GTPases是肌动蛋白细胞骨架动力学的主要调节剂，并影响细胞的形状和迁移。几种Rho GTP酶的表达通常在肿瘤中上调，这通常与患者的不良预后相关。癌细胞通过排列在血管中的内皮细胞的迁移，称为跨内皮迁移或外渗，是转移过程中的关键步骤。使用siRNA技术特异性靶向每个Rho家族成员，结合成像技术，可以研究单个Rho GTP酶的作用。在这一章中，我们描述的方法来评估Rho GTP酶如何影响体外癌细胞跨内皮细胞迁移的不同步骤。
Small Rho GTPases are major regulators of actin cytoskeleton dynamics and influence cell shape and migration. The expression of several Rho GTPases is often up-regulated in tumors and this frequently correlates with a poor prognosis for patients. Migration of cancer cells through endothelial cells that line the blood vessels, called transendothelial migration or extravasation, is a critical step during the metastasis process. The use of siRNA technology to target specifically each Rho family member coupled with imaging techniques allows the roles of individual Rho GTPases to be investigated. In this chapter we describe methods to assess how Rho GTPases affect the different steps of cancer cell transendothelial cell migration in vitro.