Preliminary success using hydrogen peroxide to treat Atlantic salmon, Salmo salar L., affected with experimentally induced amoebic gill disease (AGD)
Preliminary success using hydrogen peroxide to treat Atlantic salmon, Salmo salar L., affected with experimentally induced amoebic gill disease (AGD)
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DOI：
10.1111/j.1365-2761.2012.01422.x
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发表时间：
2012-11-01
影响因子：
2.5
通讯作者：
Nowak, B. F.
中科院分区：
文献类型：
--
作者：
Adams, M. B.;Crosbie, P. B. B.;Nowak, B. F.
目前，唯一有效的和商业上使用的治疗阿米巴鳃病（AGD）在养殖塔斯马尼亚大西洋鲑鱼是淡水洗澡。过氧化氢（H2 O2），通常用于整个水产养殖业的一系列局部皮肤和鳃感染，在体外和体内试验，以确定其作为替代治疗AGD的潜力。在体外条件下，滋养体的Neoparamoeba perurans暴露于三个浓度的H2 O2在海水中（500，1000和1500毫克L-1）超过四个持续时间（10，20，30和60分钟），每个在两个温度（12和18摄氏度）。暴露后立即和24 h后评估滋养体活力。1000 mg L-1的浓度/持续时间组合>10 min显示出有效的杀阿米巴活性。随后，大西洋鲑鱼轻度影响实验诱导AGD处理过氧化氢在12和18摄氏度15分钟，在1250毫克L-1和他们的再感染率相比，淡水处理的鱼超过21天。浴后立即观察到处理组之间受增生性病变（与阿米巴病相关）影响的细丝百分比和血浆渗透压的显著差异。然而，在治疗后3周内的疾病消退方面，结果在很大程度上是不明确的。这些数据表明，在实验室条件下，H2 O2治疗海水中成功地改善了AGD的临床轻病例。
Currently, the only effective and commercially used treatment for amoebic gill disease (AGD) in farmed Tasmanian Atlantic salmon is freshwater bathing. Hydrogen peroxide (H2O2), commonly used throughout the aquaculture industry for a range of topical skin and gill infections, was trialled in vitro and in vivo to ascertain its potential as an alternative treatment against AGD. Under in vitro conditions, trophozoites of Neoparamoeba perurans were exposed to three concentrations of H2O2 in sea water (500, 1000 and 1500 mg L-1) over four durations (10, 20, 30 and 60 min) each at two temperatures (12 and 18 degrees C). Trophozoite viability was assessed immediately post-exposure and after 24 h. A concentration/duration combination of 1000 mg L-1 for >10 min demonstrated potent amoebicidal activity. Subsequently, Atlantic salmon mildly affected with experimentally induced AGD were treated with H2O2 at 12 and 18 degrees C for 15 min at 1250 mg L-1 and their re-infection rate was compared to freshwater-treated fish over 21 days. Significant differences in the percentage of filaments affected with hyperplastic lesions (in association with amoebae) and plasma osmolality were noted between treatment groups immediately post-bath. However, the results were largely equivocal in terms of disease resolution over a 3-week period following treatment. These data suggest that H2O2 treatment in sea water successfully ameliorated a clinically light case of AGD under laboratory conditions.