MASS-SPECTROMETRY OF A HUMAN-TUMOR GLYCOLIPID ANTIGEN BEING DEFINED BY MOUSE MONOCLONAL-ANTIBODY NS-19-9
MASS-SPECTROMETRY OF A HUMAN-TUMOR GLYCOLIPID ANTIGEN BEING DEFINED BY MOUSE MONOCLONAL-ANTIBODY NS-19-9
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DOI：
10.1016/0006-291x(83)91160-9
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
KOPROWSKI, H
中科院分区：
文献类型：
--
作者：
FALK, KE;KARLSSON, KA;KOPROWSKI, H
用抗体-色谱结合试验跟踪制备，使用对胃肠道肿瘤具有明显特异性的小鼠单克隆抗体从人胰腺癌中分离糖脂。3种衍生物的直接探针质谱确定了糖序列为NeuAc-己糖-（岩藻糖）N-乙酰己糖胺-己糖-己糖，神经酰胺主要由植物鞘氨醇和16-24碳2-羟基脂肪酸组成。2个衍生物的NMR光谱可能存在该序列。Gal.beta.→3G1cNAc（4.rarw. 1.alpha. Fuc）β 1 →，也就是刘易斯a血型的决定因素这与最近的降解研究结果一致。
With an antibody-to-chromatogram binding assay to follow the preparation a glycolipid was isolated from human pancreatic carcinoma using a mouse monoclonal antibody of apparent specificity for gastrointestinal tumors. Direct probe mass spectrometry of 3 derivatives established the sugar sequence as NeuAc-hexose-(fucose)N-acetylhexosamine-hexose-hexose and the ceramide to be composed mainly of phytosphingosine and 16-24 carbon 2-hydroxy fatty acids. NMR spectroscopy of 2 of the derivatives made likely the presence of the sequence. Gal.beta. .fwdarw. 3G1cNAc (4 .rarw. 1.alpha. Fuc).beta.1 .fwdarw., which is the blood group Lewis a determinant. This is in agreement with recent results from degradation studies.