Gall Wasp Transcriptomes Unravel Potential Effectors Involved in Molecular Dialogues With Oak and Rose
Gall Wasp Transcriptomes Unravel Potential Effectors Involved in Molecular Dialogues With Oak and Rose
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DOI：
10.3389/fphys.2019.00926
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发表时间：
2019-07-24
影响因子：
4
通讯作者：
Drezen, Jean-Michel
中科院分区：
文献类型：
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作者：
Cambier, Sebastien;Ginis, Olivia;Drezen, Jean-Michel
为了深入了解可能参与木本植物初始诱导瘿虫的黄蜂因子，我们对两种分别在橡树和玫瑰上诱导瘿虫的木瘿蜂Biorhiza pellicle和Diplolepis rosae的卵巢和毒液腺进行了高通量（454）转录组分析。将从头组装和注释的contigs与系统发育相关的拟寄生蜂的序列进行比较。估计contigs的相对表达水平，以确定每个组织中表达最多的基因序列。我们首次鉴定了一组母系表达的瘿蜂蛋白可能参与与植物的相互作用。一些在毒腺和卵巢中高表达的基因可能抑制植物早期防御信号。我们还发现瘿蜂纤维素酶可能参与观察到的植物组织在产卵后的局部裂解，并且可能通过水平基因转移从细菌中获得。我们没有发现病毒相关基因表达的证据，与许多非犬皮类寄生蜂相反。通过对瘿蜂效应的探索，本研究是了解木本植物次生瘿蜂诱导分子机制的第一步，而最近对橡树和玫瑰基因组的测序将有助于研究植物对这些因素的反应。
To gain insight into wasp factors that might be involved in the initial induction of galls on woody plants, we performed high throughput (454) transcriptome analysis of ovaries and venom glands of two cynipid gall wasps, Biorhiza pellicle and Diplolepis rosae, inducing galls on oak and rose, respectively. De novo assembled and annotated contigs were compared to sequences from phylogenetically related parasitoid wasps. The relative expression levels of contigs were estimated to identify the most expressed gene sequences in each tissue. We identify for the first time a set of maternally expressed gall wasp proteins potentially involved in the interaction with the plant. Some genes highly expressed in venom glands and ovaries may act to suppress early plant defense signaling. We also identify gall wasp cellulases that could be involved in observed local lysis of plant tissue following oviposition, and which may have been acquired from bacteria by horizontal gene transfer. We find no evidence of virus-related gene expression, in contrast to many non-cynipid parasitoid wasps. By exploring gall wasp effectors, this study is a first step toward understanding the molecular mechanisms underlying cynipid gall induction in woody plants, and the recent sequencing of oak and rose genomes will enable study of plant responses to these factors.