The Impact of Oxidative Stress Levels on the Clinical Effectiveness of Sivelestat in Treating Acute Lung Injury: An Electron Spin Resonance Study
The Impact of Oxidative Stress Levels on the Clinical Effectiveness of Sivelestat in Treating Acute Lung Injury: An Electron Spin Resonance Study
复制标题
DOI：
10.1097/ta.0b013e3181bb80d4
复制
发表时间：
2010-04-01
影响因子：
--
通讯作者：
Yokoi, Isao
中科院分区：
文献类型：
--
作者：
Matsumoto, Shigekiyo;Shingu, Chihiro;Yokoi, Isao
背景：西维司他是一种中性粒细胞弹性酶抑制剂，已被用于治疗急性肺损伤（ALI），并取得了不同程度的临床成功。ALI患者不同的氧化应激基线水平被认为是结果不一致的一个解释。方法：应用床边电子自旋共振谱仪，对ALI患者血清抗坏血酸自由基添加二甲亚砜（AFR/DMSO）后的电子自旋共振信号强度进行评价。结果：我们发现AFR/DMSO与抗坏血酸水平呈正相关，表明血清AFR/DMSO测量可以作为实时评估氧化应激的替代指标。ALI患者的AFR/DMSO水平明显低于健康对照组。分层分析显示，与对西司他无效的ALI患者相比，基线AFR/DMSO水平明显较低。结论：我们的研究结果表明，西司他的临床疗效取决于基线氧化应激水平。
Background: Sivelestat, a neutrophil elastase inhibitor, has been used to treat acute lung injury (ALI) with varying levels of clinical success. Variable baseline levels of oxidative stress in patients with ALI have been proposed as one explanation for inconsistent results.Methods: Using a bedside electron spin resonance spectrometer, we evaluated electron spin resonance signal intensities of serum ascorbyl free radicals supplemented with dimethyl sulfoxide (AFR/DMSO) in patients with ALI.Results: We found a positive correlation between AFR/DMSO and ascorbate levels, suggesting that serum AFR/DMSO measurements may serve as a surrogate for real-time assessments of oxidative stress. Levels of AFR/DMSO in patients with ALI were significantly lower than those found in healthy controls. Stratified analyses revealed that baseline AFR/DMSO levels were significantly lower in patients with ALI who failed to respond to sivelestat compared with those who did respond.Conclusions: Our results suggest that the clinical efficacy of sivelestat is dependent on baseline oxidative stress levels.