The genomic architecture of mastitis resistance in dairy sheep.
The genomic architecture of mastitis resistance in dairy sheep.
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DOI：
10.1186/s12864-017-3982-1
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发表时间：
2017-08-16
期刊：
影响因子：
4.4
通讯作者：
Psifidi A
中科院分区：
文献类型：
--
作者：
Banos G;Bramis G;Bush SJ;Clark EL;McCulloch MEB;Smith J;Schulze G;Arsenos G;Hume DA;Psifidi A
乳腺炎是奶牛最常见的疾病，具有重大的经济、卫生和福利影响。尽管实施了改进的管理措施，但该病在所有奶羊生产系统中仍然存在。选择性育种以增强乳腺炎抗性可提供进一步控制疾病的手段。在本研究中，我们调查了奶牛乳房炎的四个性状的遗传结构。个体动物记录临床乳腺炎的发生和三个乳腺炎指标性状（牛奶体细胞计数，总活菌数在牛奶和加州乳腺炎测试），每月收集整个哺乳期的609只母羊的希腊希奥斯品种。所有的动物基因型与定制的960-单核苷酸多态性（SNP）DNA阵列的基础上位于数量性状位点（QTL）区域的标记乳腺炎抗性先前在其他三个不同的奶牛群体检测。对临床型乳房炎发生率和三个乳房炎指标性状的遗传变异和强正遗传相关进行了估计。在染色体2、3、5、16和19上证实了与这些乳腺炎性状显著相关的SNP标记。我们确定了乳腺炎抗性的途径、分子相互作用网络和功能基因簇。基于绵羊转录图谱和来自乳体细胞的转录组数据的分析，鉴定了检测区域内的候选基因。涉及先天免疫的相关候选基因包括SOCS 2、CTLA 4、C6、C7、C9、PTGER 4、DAB 2、CARD 6、OSMR、PLXNC 1、IDH 1、ICOS、FYB和LYFR。结果证实了存在的动物遗传变异性乳腺炎的抗性和确定的基因组区域与特定的乳腺炎性状的希奥斯羊。不同的奶牛品种之间的乳腺炎抗性的保守的遗传结构表明，跨品种选择方案是可行的。本文的在线版本（doi：10.1186/s12864-017-3982-1）包含补充材料，可供授权用户使用。
Mastitis is the most prevalent disease in dairy sheep with major economic, hygienic and welfare implications. The disease persists in all dairy sheep production systems despite the implementation of improved management practises. Selective breeding for enhanced mastitis resistance may provide the means to further control the disease. In the present study, we investigated the genetic architecture of four mastitis traits in dairy sheep. Individual animal records for clinical mastitis occurrence and three mastitis indicator traits (milk somatic cell count, total viable bacterial count in milk and the California mastitis test) were collected monthly throughout lactation for 609 ewes of the Greek Chios breed. All animals were genotyped with a custom-made 960-single nucleotide polymorphism (SNP) DNA array based on markers located in quantitative trait loci (QTL) regions for mastitis resistance previously detected in three other distinct dairy sheep populations. Heritable variation and strong positive genetic correlations were estimated for clinical mastitis occurrence and the three mastitis indicator traits. SNP markers significantly associated with these mastitis traits were confirmed on chromosomes 2, 3, 5, 16 and 19. We identified pathways, molecular interaction networks and functional gene clusters for mastitis resistance. Candidate genes within the detected regions were identified based upon analysis of an ovine transcriptional atlas and transcriptome data derived from milk somatic cells. Relevant candidate genes implicated in innate immunity included SOCS2, CTLA4, C6, C7, C9, PTGER4, DAB2, CARD6, OSMR, PLXNC1, IDH1, ICOS, FYB, and LYFR. The results confirmed the presence of animal genetic variability in mastitis resistance and identified genomic regions associated with specific mastitis traits in the Chios sheep. The conserved genetic architecture of mastitis resistance between distinct dairy sheep breeds suggests that across-breed selection programmes would be feasible. The online version of this article (doi:10.1186/s12864-017-3982-1) contains supplementary material, which is available to authorized users.
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影响因子：
5.4
作者：
Freeman TC;Ivens A;Baillie JK;Beraldi D;Barnett MW;Dorward D;Downing A;Fairbairn L;Kapetanovic R;Raza S;Tomoiu A;Alberio R;Wu C;Su AI;Summers KM;Tuggle CK;Archibald AL;Hume DA
通讯作者：
Hume DA
影响因子：
3.7
作者：
Gutiérrez-Gil B;Arranz JJ;Pong-Wong R;García-Gámez E;Kijas J;Wiener P
通讯作者：
Wiener P
影响因子：
4.8
作者：
Fujino, H;West, KA;Regan, JW
通讯作者：
Regan, JW
DOI：
10.1111/j.1439-0442.1991.tb01034.x
发表时间：
1991-07-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE
影响因子：
--
作者：
GIRI, SN;STABENFELDT, GH;OSBURN, BI
通讯作者：
OSBURN, BI
影响因子：
14.9
作者：
Cunningham F;Amode MR;Barrell D;Beal K;Billis K;Brent S;Carvalho-Silva D;Clapham P;Coates G;Fitzgerald S;Gil L;Girón CG;Gordon L;Hourlier T;Hunt SE;Janacek SH;Johnson N;Juettemann T;Kähäri AK;Keenan S;Martin FJ;Maurel T;McLaren W;Murphy DN;Nag R;Overduin B;Parker A;Patricio M;Perry E;Pignatelli M;Riat HS;Sheppard D;Taylor K;Thormann A;Vullo A;Wilder SP;Zadissa A;Aken BL;Birney E;Harrow J;Kinsella R;Muffato M;Ruffier M;Searle SM;Spudich G;Trevanion SJ;Yates A;Zerbino DR;Flicek P
通讯作者：
Flicek P