Sex-specific interactions affect foliar defense compound accumulation and resistance to herbivores in Populus cathayana
Sex-specific interactions affect foliar defense compound accumulation and resistance to herbivores in Populus cathayana
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性别特异性相互作用影响青杨叶防御化合物的积累和对食草动物的抵抗力
DOI：
10.1016/j.scitotenv.2021.145819
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发表时间：
2021-02-19
影响因子：
9.8
通讯作者：
Xia, Zhichao
中科院分区：
文献类型：
--
作者：
He, Yue;Zhu, Zuodong;Xia, Zhichao
雌雄异株植物的两性异形现象普遍存在，但两性间的互作对雌雄植物生长和抗虫性的影响尚不清楚。为了研究青杨雌株（F）和雄株（M）与相同（F/F，M/M）或异性（F/M）邻居在不同草食动物处理（无草食动物和有草食动物）下的性别特异性相互作用。我们发现，女性的反应，男性增加拍摄质量下的存在和不存在的食草动物。与此相反，男性响应女性的地上部生物量的增加，只有在草食动物的存在下。食草动物增加了两性叶片中的酚类物质的产生，但更强烈的女性。此外，茉莉酸（JA）的生产在女性的草食动物的存在下，而不是在男性增强。田间试验表明，女性在一个纯粹的女性种植园（PF）有更大的总酚含量的叶片相比，男性在一个纯粹的男性种植园（PM），最终导致较低的损害草食动物喂养。叶片损害的男性减少在混合性别种植园（MS）相比，PM，但有PM和MS之间的女性没有差异。代谢组学分析突出了显着差异，在叶片之间的代谢谱男性和女性之间的协议，观察到的差异，草食动物的抗性。雌性和雄性的代谢组也不同纯性种植（PF或PM）和MS之间，而没有显着差异，在MS之间的女性和男性。因此，MS中的雄性草食动物摄食的减少不仅与雌性的联合作用有关，而且还依赖于生化调节。本研究表明，在性别特异性的相互作用的草食动物的反应的主要差异。这一新的认识为提高雌雄异株植物人工林的抗虫性提供了新的机会。(C)2021爱思唯尔有限公司版权所有。
Sexual dimorphism is common in dioecious plants, but the effects of sex-specific interactions on the growth and herbivore resistance of females and males are still unknown. To assess sex-specific interactions, Populus cathayana females (F) and males (M) were grown in a common garden experiment with the same (F/F, M/M) or opposite sex neighbors (F/M) under different herbivory treatments (no herbivores vs. with herbivores). We found that females responded to males by increasing shoot mass under both the presence and absence of herbivores. In contrast, males responded to females by increasing shoot biomass only under the presence of herbivores. Herbivory increased phenolic production in the leaves of both sexes, but more strongly in females. In addition, jasmonic acid (JA) production was enhanced in females under the presence of herbivores, but not in males. Field experiments showed that females in a pure female plantation (PF) had greater total phenolic contents of leaves compared to males in a pure male plantation (PM), ultimately resulting in lower damage by herbivore feeding. Leaf damage of males was reduced in a mixed-sex plantation (MS) compared to PM, but there were no differences in females between PM and MS. Metabolomics analyses highlighted significant differences in leaf metabolic profiles between males and females in agreement with observed differences in herbivore resistance. Female and male metabolomes also differed between pure sex plantations (PF or PM) and MS, while no significant differences were detected in MS between females and males. Thus, the reduction of herbivore feeding in males in MS was not only related to the associational effects of females, but also depended on biochemical adjustments. The present study demonstrated major differences in sex-specific interactions in responses to herbivores. This new knowledge provides novel opportunities for the increase of insect resistance of plantations composed of dioecious plants. (C) 2021 Elsevier B.V. All rights reserved.