Sperm chemotaxis in siphonophores: Identification and biochemical properties of the attractant
Sperm chemotaxis in siphonophores: Identification and biochemical properties of the attractant
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管水母中精子的趋化性：引诱剂的鉴定和生化特性
DOI：
10.1002/cm.970060222
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
M. Cosson
中科院分区：
文献类型：
--
作者：
J. Cosson;D. Carré;M. Cosson
材料和方法春季在法国苏梅尔湾采集了河马管水母类的标本，如前所述获得了配子和壳状体[CarrC等，19811]。杯状物的大小范围为0.12 mm直径。将聚丙烯酰胺梯度凝胶[Neuhoff，19731]制备在总体积为5pl(长32 mm，直径0.4 mm)的微毛细血管内(红色布劳布兰德内部)。毛细血管通过吸入0.35M三硫酸盐(Sigma，St.Louis)pH 8.8，0.37%TEMED和0.75%过硫酸铵(Biorad)的溶液一半填充。然后，用40%丙烯酰胺(SERVA)、0.8%双丙烯酰胺、0.35M三硫酸盐(pH 8.8)和0.37%TEMED的溶液吸入每根毛细血管的后半部分。用0.05M Tris-base和0.38M甘氨酸(SERVA)组成的运行缓冲液，在60V下运行45min。这些凝胶是从
MATERIALS AND METHODSSpecimens of the siphonophore Hippopodius hippopus were collected during spring in the bay of Villefranche-sur-mer (France), and gametes and cupules were obtained as described earlier [CarrC et al, 19811. Sizes of the cupules range 0.12 mm diameter. Polyacrylamide gradient gels [Neuhoff, 19731 were prepared inside microcapillaries (Intra-red Blaubrand) of 5 pl total volume (32 mm length and 0.4 mm diameter). Capillaries were half filled by suction of a solution of 0.35 M Tris-sulfate (Sigma, St. Louis) pH 8.8, 0.37% TEMED, and 0.75% ammonium persulfate (Biorad). Then, the second half of each capillary was filled by suction of a solution of 40% acrylamide (Serva), 0.8% bis-acrylamide, 0.35 M Tris-sulfate pH 8.8, and 0.37% TEMED. Electrophoreses were run for 45 min at 60 V with running buffers composed of 0.05 M Tris-base, 0.38 M glycine (Serva). The gels were extruded from
与卵子相关的肽会导致精子主要质膜蛋白的迁移率发生变化。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Suzuki,N;Shimomura,H;Radany,EW;Ramarao,CS;Ward,GE;Bentley,JK;Garbers,DL
通讯作者：
Garbers,DL